DYKDDDDK (binds to Sigma FLAG®) (rabbit antibody, polyclonal)

AS15 3037 | Clonality: Polyclonal | Host: Rabbit | Reactivity: DYKDDDDK-tag

Product Information

Immunogen

KLH-conjugated synthetic peptide: DYKDDDDK (Sigma FLAG®).

Host Rabbit

Clonality Polyclonal

Purity Affinity purified

Format Liquid in PBS, pH 7.4, with 0.05% sodium azide

Quantity 100 µg

Storage Store at -20°C for up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

Tested applications Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 1000 (WB) 1 : 200 (IP), 1 : 200 (IHC)

Reactivity

Confirmed reactivity DYKDDDDK epitope tag (Sigma FLAG®), fused to proteins in mammalian cells

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information Exact working dilution needs to be determined by end user.

Related products

AS15 2871 | anti-DYKDDDDK (binds to Sigma FLAG®), mouse monoclonal antibody

AS15 2952 | anti-DYKDDDDK (binds to Sigma FLAG®), ALP conjugated, mouse monoclonal antibody

AS15 2950 | anti-DYKDDDDK (binds to Sigma FLAG®), DyLight® 488 conjugated (mouse antibody)

AS15 2951 | anti-DYKDDDDK (binds to Sigma FLAG®), DyLight® 550 conjugated (mouse antibody)

AS15 2947 | anti-DYKDDDDK (binds to Sigma FLAG®), DyLight® 650 conjugated (mouse antibody)

AS15 2948 | anti-DYKDDDDK (binds to Sigma FLAG®), Europium conjugated (mouse antibody)

AS15 2949 | anti-DYKDDDDK (binds to Sigma FLAG®), SureLight® R-Phycoerythrin conjugated (mouse antibody)

AS15 3036 | anti-DYKDDDDK (binds to Sigma FLAG®), (mouse antibody)

other tag antibodies

Secondary antibodies

Background

Rabbit anti-DYKDDDDK is a primary antibody which binds to DYKDDDDK (Sigma FLAG®).

Product citations

Selected references Salesse-Smith et al. (2018). Overexpression of Rubisco subunits with RAF1 increases Rubisco content in maize. Nat Plants. 2018 Oct;4(10):802-810. doi: 10.1038/s41477-018-0252-4.

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