IKP

DYKDDDDK (binds to Sigma FLAG®) (rabbit antibody, polyclonal)

Product no: AS15 3037

AS15 3037  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: DYKDDDDK-tag

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  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide: DYKDDDDK (Sigma FLAG®).

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified in PBS, pH 7.4. Contains 0.055% sodium azide.
    Format: Liquid
    Quantity: 100 µg
    Storage: Store at -20°C for up to 1 year, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB)
    Recommended dilution: 1 : 1000 (WB) 1 : 200 (IP), 1 : 200 (IHC)
  • Reactivity
  • Confirmed reactivity: DYKDDDDK epitope tag (Sigma FLAG®), fused to proteins in plant and mammalian cells
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Background
  • Background:

    Rabbit anti-DYKDDDDK is a primary antibody which binds to DYKDDDDK (Sigma FLAG®).

  • Product Citations
  • Selected references: Salesse-Smith et al. (2018). Overexpression of Rubisco subunits with RAF1 increases Rubisco content in maize. Nat Plants. 2018 Oct;4(10):802-810. doi: 10.1038/s41477-018-0252-4.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials
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