DYKDDDDK (binds to Sigma FLAGŪ) (rabbit antibody, polyclonal)
AS15 3037 | Clonality: Polyclonal | Host: Rabbit | Reactivity: DYKDDDDK-tag
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Product Information
Immunogen
KLH-conjugated synthetic peptide: DYKDDDDK (Sigma FLAG®).
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified in PBS, pH 7.4. Contains 0.055% sodium azide.
Format
Liquid
Quantity
100 ĩg
Storage
Store at -20°C for up to 1 year, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1 : 1000 (WB) 1 : 200 (IP), 1 : 200 (IHC)
Reactivity
Confirmed reactivity
DYKDDDDK epitope tag (Sigma FLAG®), fused to proteins in plant and mammalian cells
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Additional information
Background
Background
Rabbit anti-DYKDDDDK is a primary antibody which binds to DYKDDDDK (Sigma FLAG®).
Product citations
Selected references
Salesse-Smith et al. (2018). Overexpression of Rubisco subunits with RAF1 increases Rubisco content in maize. Nat Plants. 2018 Oct;4(10):802-810. doi: 10.1038/s41477-018-0252-4.
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