DYKDDDDK (binds to Sigma FLAGŪ) (rabbit antibody, polyclonal)
AS15 3037 | Clonality: Polyclonal | Host: Rabbit | Reactivity: DYKDDDDK-tag
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Product Information
Immunogen
KLH-conjugated synthetic peptide: DYKDDDDK (Sigma FLAG®).
Host
Rabbit
Clonality
Polyclonal
Purity
Affinity purified
Format
Liquid in PBS, pH 7.4, with 0.05% sodium azide
Quantity
100 µg
Storage
Store at -20°C for up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Tested applications
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1 : 1000 (WB) 1 : 200 (IP), 1 : 200 (IHC)
Reactivity
Confirmed reactivity
DYKDDDDK epitope tag (Sigma FLAG®), fused to proteins in mammalian cells
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Application examples
Additional information
Additional information
Exact working dilution needs to be determined by end user.
Background
Background
Rabbit anti-DYKDDDDK is a primary antibody which binds to DYKDDDDK (Sigma FLAG®).
Product citations
Selected references
Salesse-Smith et al. (2018). Overexpression of Rubisco subunits with RAF1 increases Rubisco content in maize. Nat Plants. 2018 Oct;4(10):802-810. doi: 10.1038/s41477-018-0252-4.
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