DYKDDDDK (binds to Sigma FLAGŪ), HRP conjugated (Clone M2)
AS16 3222 | Clonality: monoclonal | Host: Mouse | Reactivity: DYKDDDDK-tag

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Product Information
Immunogen
DYKDDDDK (Sigma FLAG®)
Host
Mouse
Clonality
Monoclonal
Purity
Protein A purified
Format
Lyophilized
Quantity
100 µg
Reconstitution
For reconstitution add 100 µl of sterile water.
Storage
Store freeze-dried poweder at 2-8°C.
Rehydrate with 1.0 ml of deionized water and let stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Shelf life: 1 year from date of receipt. Prepare working dilution prior to use and then discard.
Tested applications
Western blot (WB)
Recommended dilution
100 ng/ml (ELISA), 1 : 20-1 : 50 (ICC), 1 : 20-1 : 50 (IF), 3 µg (IP), 1 : 10-1 : 50 (WB)
Reactivity
Application examples
Additional information
Additional information
Antibody is provided in: 100 mM NaPO4 (pH 7.4), 100 mM NaCl, 1.5% BSA, 50 mM Sucrose, 0.01 % Thimerosal.
Affinity purified antibody has over 95% purity based on SDS-PAGE.Background
Background
Mouse anti-DYKDDDDK is a primary antibody which binds to DYKDDDDK (Sigma FLAG®) and is directly conjugated to HRP, Horseradish Peroxidase. This is of advantage to shorten assay time by no need to use a secondary antibody to DYKDDDDK.
Product citations
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