DYKDDDDK (binds to Sigma FLAG®), HRP conjugated (Clone M2)

AS16 3222 | Clonality: monoclonal | Host: Mouse | Reactivity: DYKDDDDK-tag

Product Information

Immunogen

DYKDDDDK (Sigma FLAG®)

Host Mouse

Clonality Monoclonal

Purity Protein A purified

Format Lyophilized

Quantity 100 µg

Reconstitution For reconstitution add 100 µl of sterile water.

Storage

Store freeze-dried poweder at 2-8°C.
Rehydrate with 1.0 ml of deionized water and let stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Shelf life: 1 year from date of receipt. Prepare working dilution prior to use and then discard.

Tested applications Western blot (WB)

Recommended dilution 100 ng/ml (ELISA), 1 : 20-1 : 50 (ICC), 1 : 20-1 : 50 (IF), 3 µg (IP), 1 : 10-1 : 50 (WB)

Reactivity

Application examples

Additional information

Antibody is provided in: 100 mM NaPO4 (pH 7.4), 100 mM NaCl, 1.5% BSA, 50 mM Sucrose, 0.01 % Thimerosal.

Affinity purified antibody has over 95% purity based on SDS-PAGE.

Related products

Related products AS15 2952 | Anti-DYKDDDDK (binds to Sigma FLAG®), ALP conjugated, mouse antibodies

AS15 2950 | Anti-DYKDDDDK (binds to Sigma FLAG®), DyLight® 488 conjugated (mouse antibody)

AS15 2951 | Anti-DYKDDDDK (binds to Sigma FLAG®), DyLight® 550 conjugated (mouse antibody)

AS15 2947 | Anti-DYKDDDDK (binds to Sigma FLAG®), DyLight® 650 conjugated (mouse antibody)

AS15 2948 | Anti-DYKDDDDK (binds to Sigma FLAG®), Europium conjugated (mouse antibody)

AS15 2949 | Anti-DYKDDDDK (binds to Sigma FLAG®), SureLight® R-Phycoerythrin conjugated (mouse antibody)

Non-conjugated anti-DYKDDDDK (binds to Sigma FLAG®) antibodies:

AS15 3036 | Anti-DYKDDDDK (binds to Sigma FLAG®), (mouse antibody)

AS15 3037 | Anti-DYKDDDDK (binds to Sigma FLAG®), (rabbit antibody)

other tag antibodies

Background

Background Mouse anti-DYKDDDDK is a primary antibody which binds to DYKDDDDK (Sigma FLAG®) and is directly conjugated to HRP, Horseradish Peroxidase. This is of advantage to shorten assay time by no need to use a secondary antibody to DYKDDDDK.