DYKDDDDK (binds to Sigma FLAG®), HRP conjugated (Clone M2)

Product no: AS16 3222
AS16 3222  | Clonality: monoclonal |  Host: Mouse | Reactivity: DYKDDDDK-tag
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  • Product Info
  • Immunogen:

    DYKDDDDK (Sigma FLAG®)

    Host: Mouse
    Clonality: Monoclonal
    Purity: Protein A purified.
    Format: Lyophilized
    Quantity: 100 µg
    Reconstitution: For reconstitution add 100 µl of sterile water
    Storage: Store freeze-dried poweder at 2-8°C.Rehydrate with 1.0 ml of deionized water and let stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily. Shelf life: 1 year from date of receipt. Prepare working dilution prior to use and then discard.
    Tested applications: Western blot (WB)
    Recommended dilution: 100 ng/ml (ELISA), 1 : 20-1 : 50 (ICC), 1 : 20-1 : 50 (IF), 3 µg (IP), 1 : 10-1 : 50 (WB)
  • Additional Information
  • Additional information: Antibody is provided in: 100 mM NaPO4 (pH 7,4), 100 mM NaCl, 1,5% BSA, 50 mM Sucrose, 0,01 % Thimerosal,Affinity purified antibody has over 95% purity based on SDS-PAGE
  • Background
  • Background: Mouse anti-DYKDDDDK is a primary antibody which binds to DYKDDDDK (Sigma FLAG®) and is directly conjugated to HRP, Horseradish Peroxidase. This is of advantage to shorten assay time by no need to use a secondary antibody to DYKDDDDK.
  • Product Citations
  • Selected references: 38221900
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