His-tag | 6xHis (polyclonal)
AS19 4255 | Clonality: polyclonal | Host: Chicken | Reactivity: His-tag
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Product Information
Immunogen
KLH-conjugated synthetic peptide 6xHis
Host
Chicken
Clonality
Polyclonal
Purity
Affinity purified in PBS pH 7.2
Format
Liquid
Quantity
100 µg
Storage
Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications
ELISA (ELISA), Western blot (WB)
Recommended dilution
1: 8000 (ELISA), 1: 1000 (WB)
Reactivity
Confirmed reactivity
6xHis
Predicted reactivity
6xHis
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Additional information
Additional information
0.1 % sodium azide is present as preservative
Background
Background
His-Tag is a polyhistidine tag which consists of 6 histidine residues introduced on N- or C-terminus of the protein. The polyhistidine-tag can be used for recombinant protein detection using specific antibodies and it is not conjugated to any dye or enzyme.
Product citations
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