Goat anti-Chicken IgY (H&L), ALP conjugated

Name: Goat anti-Chicken IgY (H&L), ALP conjugated
SKU: AS09 606
Price: 206 EUR
Availability: InStock

AS09 606 | Clonality: Polyclonal | Host: Goat | Reactivity: Chicken IgY (H&L)

10 % until end of 2023. Use discount code: Conj10

Goat anti-Chicken IgY (H&L), ALP conjugated in the group Secondary Antibodies / Anti-Chicken / ALP (alkaline phosphatase) at Agrisera AB (Antibodies for research) (AS09 606)

Product Information

Immunogen

purified chicken IgY, whole molecule

Host Goat

Clonality Polyclonal

Purity Immunogen affinity purified goat IgG.

Format Liquid

Quantity 1 mg

Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Tested applications ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 10 000 -1 : 100 000 (ELISA), 1 : 500 -1 : 2000 (IHC), 1 : 10 000-100 000 (WB)

Reactivity

Confirmed reactivity Chicken IgY heavy and light chains (H&L)

Predicted reactivity Chicken IgY Heavy and Light chains (H&L)

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Concentration: 1.50 mg/ml (E 1% at 280 nm = 13.0)

Antibody concentration is 1.14 mg/ml and it has been afinity purified on solid phase hen IgY (H&L)

Antibody is affinity purified using solid phase Chicken IgG/Y.

AP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative

No reactivity is observed to non-immunoglobulin hen serum proteins based in immunoelectrophoresis

chicken IgY is also called chicken or hen IgG

Background

Goat anti-chicken IgY is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to hen IgY immunoglobulin in immunological assays.

Product citations

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immunogen:	purified chicken IgY, whole molecule
Host:	Goat
Clonality:	Polyclonal
Purity:	Immunogen affinity purified goat IgG.
Format:	Liquid
Quantity:	1 mg
storage:	Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
tested applications:	ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
recommended dilution:	1 : 10 000 -1 : 100 000 (ELISA), 1 : 500 -1 : 2000 (IHC), 1 : 10 000-100 000 (WB)
Confirmed reactivity:	Chicken IgY heavy and light chains (H&L)
predicted reactivity:	Chicken IgY Heavy and Light chains (H&L)
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	Concentration: 1.50 mg/ml (E 1% at 280 nm = 13.0) Antibody concentration is 1.14 mg/ml and it has been afinity purified on solid phase hen IgY (H&L) Antibody is affinity purified using solid phase Chicken IgG/Y. AP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative
additional information (application):	No reactivity is observed to non-immunoglobulin hen serum proteins based in immunoelectrophoresis chicken IgY is also called chicken or hen IgG
background:	Goat anti-chicken IgY is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to hen IgY immunoglobulin in immunological assays.