Goat anti-Chicken IgY (H&L), ALP conjugated

Product no: AS09 606

AS09 606 | Clonality: Polyclonal  |  Host: Goat  | Reactivity: Chicken IgY (H&L)

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  • Product Info
  • Immunogen:

    purified chicken IgY, whole molecule

    Host: Goat
    Clonality: Polyclonal
    Purity: Immunogen affinity purified goat IgG.
    Format: Liquid
    Quantity: 1 mg
    Storage: Non-diluted antibody is stable for 4 years at 2-8C. For storage at -20C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
    Tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
    Recommended dilution: 1 : 10 000 -1 : 100 000 (ELISA), 1 : 500 -1 : 2000 (IHC), 1 : 10 000-100 000 (WB)
  • Reactivity
  • Confirmed reactivity: Chicken IgY heavy and light chains (H&L)
    Predicted reactivity: Chicken IgY Heavy and Light chains (H&L)
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information:

    Concentration: 1.50 mg/ml (E 1% at 280 nm = 13.0)

    Antibody concentration is 1.14 mg/ml and it has been afinity purified on solid phase hen IgY (H&L)

    Antibody is affinity purified using solid phase Chicken IgG/Y.

    AP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative

    Additional information (application):

    No reactivity is observed to non-immunoglobulin hen serum proteins based in immunoelectrophoresis

    chicken IgY is also called chicken or hen IgG

  • Background
  • Background:

    Goat anti-chicken IgY is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to hen IgY immunoglobulin in immunological assays.

  • Product Citations
  • Selected references: 38221900
  • Protocols
  • Agrisera Western Blot Guide
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