Goat anti-Chicken IgY (H&L), DyLight® 594 conjugated

Product no: AS11 1823

AS11 1823 | Clonality: Polyclonal  |  Host: Goat  | Reactivity: Chicken IgY (H&L)

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  • Product Info
  • Immunogen:

    purified chicken IgY, whole molecule

    Host: Goat
    Clonality: Polyclonal
    Purity: Immunogen affinity purified goat IgG.
    Format: Lyophilized
    Quantity: 1 mg
    Reconstitution: For reconstitution add 1 ml of sterile water, Allow reconstituted product to stand for at least 30 minutes at room temperature prior to dilution, If necessary, centrifuge to remove any particulates, Prepare fresh working dilution daily
    Storage: Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
    Tested applications: Immunocytochemistry (ICC), Immunohistochemistry (IHC)
    Recommended dilution: 1 : 50-1 : 5 000 (ICC), 1 : 20 -1 : 2000 (IHC)
  • Reactivity
  • Confirmed reactivity: Chicken IgY heavy and light chains (H&L)
    Predicted reactivity: Chicken IgY Heavy and Light chains (H&L)
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information:

    Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

    DyLight® 594  (Ex = 593 nm; Em = 618 nm).

    Additional information (application):

    Based on immunoelectrophoresis this antibody reacts with heavy chains on chicken IgG and light chains on all chicken immunoglobulins.

    No reactivity is observed to non-immunoglobulin chicken serum proteins based in immunoelectrophoresis. 

  • Background
  • Background:

    Goat anti-chicken IgY, DyLight® 594 Conjugate is a secondary antibody conjugated to  DyLight® 594, which binds to hen immunoglobulins in immunological assays. 

    DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

  • Product Citations
  • Selected references: 38221900
  • Protocols
  • Antibody protocols
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