aadA1 | Aminoglycoside adenyltransferase (chloroplast transformation marker)

Name: aadA1 | Aminoglycoside adenyltransferase (chloroplast transformation marker)
SKU: AS09 580
Price: 302 EUR
Availability: InStock

AS09 580 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Nicotiana tabacum, Chlamydomonas reinhardtii

aadA1 | Aminoglycoside adenyltransferase (chloroplast transformation marker) in the group Tag Antibodies / aadA1/BAR/BC2/BirA/CBP/GAL4/GUS/LUC at Agrisera AB (Antibodies for research) (AS09 580)

aadA1 | Aminoglycoside adenyltransferase (chloroplast transformation marker)

Product Information

Immunogen

KLH-conjugated peptide derived from known aadA1 protein sequences

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 50 µg

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 3 000 (WB)

Expected | apparent MW

30 | 30 kDa

Reactivity

Confirmed reactivity aadA casesette in Nicotiana tabacum, Chlamydomonas reinhardtii

Predicted reactivity

Acinetobacter baumannii, Enterobacter agglomerans, Klebsiella pneumoniae, Klebsiella oxytoca, Kluyvera ascorbata, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serovar Worthington, Staphylococcus aureus, Serratia sp., Shigella flexneri

Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application example

Chlamydomonas reinhardtii cells (1,2) (corresponding to 10 ug of chlorophyll) and soluble extracts of Nicotiana tabaccum leaves (3,4) were separated on a 15% acrylamide gel containing 8M urea, transferred on nitrocellulose membrane, incubated with anti-aad1 antibodies in 1: 5000 dilution for 1 h in RT, followed by washes and incubation with a secondary anti-rabbuit IgG antibody in 1: 10 000 dilution) and revealed by chemiluminescent detection reagent (3 min exposure). Tested Chlamydomonas reinhardtii and Nicotiana tabaccum strains expressed (+) or not (-) the aadA cassette.
AadA protein in Chlamydomonas reinhardtii migrates more slowly than its Nicotiana tabaccum counterpart as it also includes the 27 first residues of the atpA coding sequence.

Courtesy of Drs Y.Choquet, K. Wostrikoff and D. Drapier, CNRS, France

Additional information

Additional information This product can be sold containing ProClin if requested

Background

aadA - aminoglycoside 3"adenyltransferase is an enzyme with nucleotidyltransferase activity. Plastid transformation in tobacco involves expression of aadA cassette, which confers resistance to spectinomycin and streptomycin and allows for transformat selection.

Product citations

Selected references Lauersen et a. (2018). Phototrophic production of heterologous diterpenoids and a hydroxy-functionalized derivative from Chlamydomonas reinhardtii. Metab Eng. 2018 Jul 12;49:116-127. doi: 10.1016/j.ymben.2018.07.005.

immunogen:	KLH-conjugated peptide derived from known aadA1 protein sequences
Reconstitution:	For reconstitution add 50 µl of sterile water
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Format:	Lyophilized
Quantity:	50 µg
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
recommended dilution:	1 : 3 000 (WB)
calculated \| apparent molecular mass [kDa]:	30 \| 30 kDa
Confirmed reactivity:	aadA casesette in Nicotiana tabacum, Chlamydomonas reinhardtii
predicted reactivity:	Acinetobacter baumannii, Enterobacter agglomerans, Klebsiella pneumoniae, Klebsiella oxytoca, Kluyvera ascorbata, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serovar Worthington, Staphylococcus aureus, Serratia sp., Shigella flexneri Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	Application example Chlamydomonas reinhardtii cells (1,2) (corresponding to 10 ug of chlorophyll) and soluble extracts of Nicotiana tabaccum leaves (3,4) were separated on a 15% acrylamide gel containing 8M urea, transferred on nitrocellulose membrane, incubated with anti-aad1 antibodies in 1: 5000 dilution for 1 h in RT, followed by washes and incubation with a secondary anti-rabbuit IgG antibody in 1: 10 000 dilution) and revealed by chemiluminescent detection reagent (3 min exposure). Tested Chlamydomonas reinhardtii and Nicotiana tabaccum strains expressed (+) or not (-) the aadA cassette. AadA protein in Chlamydomonas reinhardtii migrates more slowly than its Nicotiana tabaccum counterpart as it also includes the 27 first residues of the atpA coding sequence. Courtesy of Drs Y.Choquet, K. Wostrikoff and D. Drapier, CNRS, France
additional information:	This product can be sold containing ProClin if requested
background:	aadA - aminoglycoside 3"adenyltransferase is an enzyme with nucleotidyltransferase activity. Plastid transformation in tobacco involves expression of aadA cassette, which confers resistance to spectinomycin and streptomycin and allows for transformat selection.
All references:	Lauersen et a. (2018). Phototrophic production of heterologous diterpenoids and a hydroxy-functionalized derivative from Chlamydomonas reinhardtii. Metab Eng. 2018 Jul 12;49:116-127. doi: 10.1016/j.ymben.2018.07.005.

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