aadA1 | Aminoglycoside adenyltransferase (chloroplast transformation marker)
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AS09 580 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Nicotiana tabacum, Chlamydomonas reinhardtii
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KLH-conjugated peptide derived from known aadA1 protein sequences
30 | 30 kDa
Acinetobacter baumannii, Enterobacter agglomerans, Klebsiella pneumoniae, Klebsiella oxytoca, Kluyvera ascorbata, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serovar Worthington, Staphylococcus aureus, Serratia sp., Shigella flexneri
Chlamydomonas reinhardtii cells (1,2) (corresponding to 10 ug of chlorophyll) and soluble extracts of Nicotiana tabaccum leaves (3,4) were separated on a 15% acrylamide gel containing 8M urea, transferred on nitrocellulose membrane, incubated with anti-aad1 antibodies in 1: 5000 dilution for 1 h in RT, followed by washes and incubation with a secondary anti-rabbuit IgG antibody in 1: 10 000 dilution) and revealed by chemiluminescent detection reagent (3 min exposure). Tested Chlamydomonas reinhardtii and Nicotiana tabaccum strains expressed (+) or not (-) the aadA cassette.
AadA protein in Chlamydomonas reinhardtii migrates more slowly than its Nicotiana tabaccum counterpart as it also includes the 27 first residues of the atpA coding sequence.
Courtesy of Drs Y.Choquet, K. Wostrikoff and D. Drapier, CNRS, France
aadA - aminoglycoside 3"adenyltransferase is an enzyme with nucleotidyltransferase activity. Plastid transformation in tobacco involves expression of aadA cassette, which confers resistance to spectinomycin and streptomycin and allows for transformat selection.
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