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Anti-aadA1 | Aminoglycoside adenyltransferase (chloroplast transformation marker)
AS09 580 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Nicotiana tabacum, Chlamydomonas reinhardtii
- Product Info
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Immunogen: KLH-conjugated peptide derived from known aadA1 protein sequences
Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum in PBS pH 7.4. Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 3 000 (WB) Expected | apparent MW: 30 | 30 kDa
- Reactivity
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Confirmed reactivity: aadA casesette in Nicotiana tabacum, Chlamydomonas reinhardtii
Predicted reactivity: Acinetobacter baumannii, Enterobacter agglomerans, Klebsiella pneumoniae, Klebsiella oxytoca, Kluyvera ascorbata, Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serovar Worthington, Staphylococcus aureus, Serratia sp., Shigella flexneri
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Application example
Chlamydomonas reinhardtii cells (1,2) (corresponding to 10 ug of chlorophyll) and soluble extracts of Nicotiana tabaccum leaves (3,4) were separated on a 15% acrylamide gel containing 8M urea, transferred on nitrocellulose membrane, incubated with anti-aad1 antibodies in 1: 5000 dilution for 1 h in RT, followed by washes and incubation with a secondary anti-rabbuit IgG antibody in 1: 10 000 dilution) and revealed by chemiluminescent detection reagent (3 min exposure). Tested Chlamydomonas reinhardtii and Nicotiana tabaccum strains expressed (+) or not (-) the aadA cassette.
AadA protein in Chlamydomonas reinhardtii migrates more slowly than its Nicotiana tabaccum counterpart as it also includes the 27 first residues of the atpA coding sequence.
Courtesy of Drs Y.Choquet, K. Wostrikoff and D. Drapier, CNRS, France - Additional Information
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Additional information: This product can be sold containing ProClin if requested - Background
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Background: aadA - aminoglycoside 3"adenyltransferase is an enzyme with nucleotidyltransferase activity. Plastid transformation in tobacco involves expression of aadA cassette, which confers resistance to spectinomycin and streptomycin and allows for transformat selection.
- Product Citations
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Selected references: Lauersen et a. (2018). Phototrophic production of heterologous diterpenoids and a hydroxy-functionalized derivative from Chlamydomonas reinhardtii. Metab Eng. 2018 Jul 12;49:116-127. doi: 10.1016/j.ymben.2018.07.005.
- Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela
Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela - Reviews:
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