showForm Anti-aadA1 | aminoglycoside adenyltransferase (chloroplast transformation marker) antibodies

Name:
Phone:
E-mail:
Message

aadA1 | Aminoglycoside adenyltransferase (chloroplast transformation marker)

50 % off on this product - use your discount code Freetag today!

AS09 580
  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Nicotiana tabacum, Chlamydomonas reinhardtii

aadA1 | Aminoglycoside adenyltransferase (chloroplast transformation marker) in the group Tag Antibodies / GAL4 / GUS / LUC / others at Agrisera AB (Antibodies for research) (AS09 580)

DATA SHEET IN PDF

Qty: 
278
Buy 2 items of this product for 206 €/items
Buy 3 items of this product for 190 €/items
How to cite this product:
Product name, number (Agrisera, Sweden)

Datasheet Product citations Protocols Add review

Product Information

Immunogen

KLH-conjugated peptide derived from known aadA1 protein sequences

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 3 000 (WB)
Expected | apparent MW

30 | 30 kDa

Reactivity

Confirmed reactivity aadA casesette in Nicotiana tabacum, Chlamydomonas reinhardtii
Predicted reactivity

Acinetobacter baumannii, Enterobacter agglomerans, Klebsiella pneumoniae, Klebsiella oxytoca, Kluyvera ascorbata, Pseudomonas aeruginosa,  Salmonella enterica subsp. enterica serovar Worthington, Staphylococcus aureus, Serratia sp., Shigella flexneri

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application example

western blot using aadA1 antibodies

Chlamydomonas reinhardtii cells (1,2) (corresponding to 10 ug  of chlorophyll) and soluble extracts of Nicotiana tabaccum leaves (3,4) were separated on a  15% acrylamide gel containing 8M urea, transferred on nitrocellulose membrane, incubated with anti-aad1 antibodies in 1: 5000 dilution for 1 h in RT, followed by washes and incubation with a secondary anti-rabbuit IgG antibody in  1: 10 000 dilution) and revealed by chemiluminescent detection reagent (3 min exposure). Tested Chlamydomonas reinhardtii and Nicotiana tabaccum strains expressed (+) or not (-) the aadA cassette.
AadA protein in Chlamydomonas reinhardtii migrates more slowly than its Nicotiana tabaccum counterpart as it also includes the 27 first residues of the atpA coding sequence.

Courtesy of Drs Y.Choquet, K. Wostrikoff and D. Drapier, CNRS, France

Additional information

Additional information This product can be sold containing ProClin if requested.

Related products

Background

Background

aadA - aminoglycoside 3"adenyltransferase is an enzyme with nucleotidyltransferase activity. Plastid transformation in tobacco involves expression of aadA cassette, which confers resistance to spectinomycin and streptomycin and allows for transformat selection. 

Product citations

Selected references Lauersen et a. (2018). Phototrophic production of heterologous diterpenoids and a hydroxy-functionalized derivative from Chlamydomonas reinhardtii. Metab Eng. 2018 Jul 12;49:116-127. doi: 10.1016/j.ymben.2018.07.005.

Related products: aadA1 | Aminoglycoside adenyltransferase (chloroplast transformation marker)

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
From 15 €
AS16 ECL-S | extreme low femtogram detection
- 10ml trial size is restricted to one unit per cust...
From 10 €
AS09 607 Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)
190 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
185 €