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BFP, GFP, YFP | Fluorescent Protein, clone 3A6

AS20 4485 | Clonality: Monoclonal | Host: Mouse | Reactivity: BFP, GFP, YFP

Offered at a 25% discount. Use discount code Tag2021

BFP, GFP, YFP | Fluorescent Protein, clone 3A6 in the group Tag Antibodies / GFP/YFP/RFP/CFP at Agrisera AB (Antibodies for research) (AS20 4485)

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Product Information

Immunogen Recombinant EBFP (NCBI accession number AX_766758 REGION: 1-717, expression vector pGEX-1N), expressed in E.coli.
Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Purity Purified on Protein A
Format

Lyophilized in a 10 mM ammonium bicarbonate buffer. Each vial contains 2 mg of BSA.

Quantity 100 µg
Reconstitution Recommended antibody concentration: 0.5 mg/ml (when dissolved at 0.5 mg/ml, the BSA concentration will be 1%).
Recommended solvent; 100 mM PBS or Tris-HCl, pH 7.0 •
Additional sodium azide ( up to 0.05%) is recommended for long term storage. •
For a 0.5 mg/ml antibody concentration in 1% BSA, dissolve in 200 μl buffer.
Storage Store lyophilized/reconstituted at 2-8°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 150-1: 3000 (ELISA), 1 : 500-1 : 5000 (WB)

Reactivity

Confirmed reactivity BFP, GFP, YFP

Application examples

Application examples ELIA cross reactivity assesment of anti-BFP, GFP, YFP antibodies


Specificity of Anti-Fluorescent Protein Immunoglobulin, clone 3A6, determined by ELISA. Antibody fraction (0.5 mg/ml) 1600X diluted in PBS containing 0,05% tween-20 and 5% non fat dry milk. Antibody was tested on various recombinant protein substrates i.e. BSA, 98% (Sigma), HIS6-hRrp4 (pET15b), GST-hRpp25 (pGEX-2T), GST-hRrp4 (pGEX-2T), HIS10-hRpp25 (pET16b), and a biotin containing peptide (22 mer).


Western blot using anti-BFP,GFP, YFP antibodies

1 µl of a total cell extract from E.coli, containing recombinant EGFP (predicted band size 27 kDa) was separated on SDS PAGE and blotted to nitrocellulose membrane. Primary antibody (0.5 mg/ml) was used at a dilution of 1: 1000 in PBS-T (0,05% tween-20) and 5% non fat dry milk. As secondary antibody anti-mouse IgG1 HRP conjugated was used and reaction was visualised with chemiluminescence, following manufacture's recommendations.

Additional information

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Background

Background Fluorescent proteins, like EBFP, can be used as protein "tags" to study the subcellular localization of proteins and/or their translocation upon stimulation and/or as markers for transfections in transient and stable expression systems.

Product citations

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