BFP, GFP, YFP | Fluorescent Protein, clone 3A6
AS20 4485 | Clonality: Monoclonal | Host: Mouse | Reactivity: BFP, GFP, YFP
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Recommended solvent; 100 mM PBS or Tris-HCl, pH 7.0 •
Additional sodium azide ( up to 0.05%) is recommended for long term storage. •
For a 0.5 mg/ml antibody concentration in 1% BSA, dissolve in 200 μl buffer.
Specificity of Anti-Fluorescent Protein Immunoglobulin, clone 3A6, determined by ELISA. Antibody fraction (0.5 mg/ml) 1600X diluted in PBS containing 0,05% tween-20 and 5% non fat dry milk. Antibody was tested on various recombinant protein substrates i.e. BSA, 98% (Sigma), HIS6-hRrp4 (pET15b), GST-hRpp25 (pGEX-2T), GST-hRrp4 (pGEX-2T), HIS10-hRpp25 (pET16b), and a biotin containing peptide (22 mer).
1 µl of a total cell extract from E.coli, containing recombinant EGFP (predicted band size 27 kDa) was separated on SDS PAGE and blotted to nitrocellulose membrane. Primary antibody (0.5 mg/ml) was used at a dilution of 1: 1000 in PBS-T (0,05% tween-20) and 5% non fat dry milk. As secondary antibody anti-mouse IgG1 HRP conjugated was used and reaction was visualised with chemiluminescence, following manufacture's recommendations.
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