BFP, GFP, YFP | Fluorescent Protein, clone 3A6

Name: BFP, GFP, YFP | Fluorescent Protein, clone 3A6
Brand: IKP
SKU: AS20 4485
Price: 241 EUR
Availability: InStock

AS20 4485 | Clonality: Monoclonal | Host: Mouse | Reactivity: BFP, GFP, YFP

BFP, GFP, YFP | Fluorescent Protein, clone 3A6 in the group Tag Antibodies / GFP/EGFP/BFP/CFP/YFP/RFP at Agrisera AB (Antibodies for research) (AS20 4485)

Product Information

Immunogen Recombinant EBFP (NCBI accession number AX_766758 REGION: 1-717, expression vector pGEX-1N), expressed in E.coli.

Host Mouse

Clonality Monoclonal

Subclass/isotype IgG1

Purity Immunoglobulin Protein A purified in a 10 mM ammonium bicarbonate buffer, with 2 mg of BSA.

Format Lyophilized

Quantity 100 µg

Reconstitution Recommended antibody concentration: 0.5 mg/ml (when dissolved at 0.5 mg/ml, the BSA concentration will be 1%).
Recommended solvent; 100 mM PBS or Tris-HCl, pH 7.0 •
Additional sodium azide ( up to 0.05%) is recommended for long term storage. •
For a 0.5 mg/ml antibody concentration in 1% BSA, dissolve in 200 μl buffer.

Storage Store lyophilized/reconstituted at 2-8°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Western blot (WB)

Recommended dilution 1 : 150-1: 3000 (ELISA), 1 : 500-1 : 5000 (WB)

Reactivity

Confirmed reactivity BFP, GFP, YFP

Application examples

Application examples ELIA cross reactivity assesment of anti-BFP, GFP, YFP antibodies

Specificity of Anti-Fluorescent Protein Immunoglobulin, clone 3A6, determined by ELISA. Antibody fraction (0.5 mg/ml) 1600X diluted in PBS containing 0,05% tween-20 and 5% non fat dry milk. Antibody was tested on various recombinant protein substrates i.e. BSA, 98% (Sigma), HIS6-hRrp4 (pET15b), GST-hRpp25 (pGEX-2T), GST-hRrp4 (pGEX-2T), HIS10-hRpp25 (pET16b), and a biotin containing peptide (22 mer).

Western blot using anti-BFP,GFP, YFP antibodies

Western blot using anti-BFP,GFP, YFP antibodies

1 µl of a total cell extract from E.coli, containing recombinant EGFP (predicted band size 27 kDa) was separated on SDS PAGE and blotted to nitrocellulose membrane. Primary antibody (0.5 mg/ml) was used at a dilution of 1: 1000 in PBS-T (0,05% tween-20) and 5% non fat dry milk. As secondary antibody anti-mouse IgG1 HRP conjugated was used and reaction was visualised with chemiluminescence, following manufacture's recommendations.

Additional information

Background

Background Fluorescent proteins, like EBFP, can be used as protein "tags" to study the subcellular localization of proteins and/or their translocation upon stimulation and/or as markers for transfections in transient and stable expression systems.

Product citations

immunogen:	Recombinant EBFP (NCBI accession number AX_766758 REGION: 1-717, expression vector pGEX-1N), expressed in E.coli.
Reconstitution:	Recommended antibody concentration: 0.5 mg/ml (when dissolved at 0.5 mg/ml, the BSA concentration will be 1%). Recommended solvent; 100 mM PBS or Tris-HCl, pH 7.0 • Additional sodium azide ( up to 0.05%) is recommended for long term storage. • For a 0.5 mg/ml antibody concentration in 1% BSA, dissolve in 200 μl buffer.
Sub class:	IgG1
Host:	Mouse
Clonality:	Monoclonal
Purity:	Immunoglobulin Protein A purified in a 10 mM ammonium bicarbonate buffer, with 2 mg of BSA.
Format:	Lyophilized
Quantity:	100 µg
storage:	Store lyophilized/reconstituted at 2-8°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Western blot (WB)
recommended dilution:	1 : 150-1: 3000 (ELISA), 1 : 500-1 : 5000 (WB)
Confirmed reactivity:	BFP, GFP, YFP
Picture (footer):	Specificity of Anti-Fluorescent Protein Immunoglobulin, clone 3A6, determined by ELISA. Antibody fraction (0.5 mg/ml) 1600X diluted in PBS containing 0,05% tween-20 and 5% non fat dry milk. Antibody was tested on various recombinant protein substrates i.e. BSA, 98% (Sigma), HIS6-hRrp4 (pET15b), GST-hRpp25 (pGEX-2T), GST-hRrp4 (pGEX-2T), HIS10-hRpp25 (pET16b), and a biotin containing peptide (22 mer). 1 µl of a total cell extract from E.coli, containing recombinant EGFP (predicted band size 27 kDa) was separated on SDS PAGE and blotted to nitrocellulose membrane. Primary antibody (0.5 mg/ml) was used at a dilution of 1: 1000 in PBS-T (0,05% tween-20) and 5% non fat dry milk. As secondary antibody anti-mouse IgG1 HRP conjugated was used and reaction was visualised with chemiluminescence, following manufacture's recommendations.
background:	Fluorescent proteins, like EBFP, can be used as protein "tags" to study the subcellular localization of proteins and/or their translocation upon stimulation and/or as markers for transfections in transient and stable expression systems.

Related products: BFP, GFP, YFP | Fluorescent Protein, clone 3A6

Agrisera ECL kit (Bright/SuperBright)

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...

From 26 €

Info

Add to cart

Blue Fluorescent Protein | BFP , clone 7F10

AS20 4486 | Clonality: Monoclonal | Host: Mouse | Reactivity: Blue Fluorescent Protein (BFP)

241 €

GFP | Green Fluorescence Protein (protein A purified)

AS15 3000 | Clonality: Polyclonal | Host: Rabbit | Reactivity: native and recombinant GFP

402 €

Goat anti-Mouse IgG1 (subclass specific), HRP conjugated

AS16 3715 | Clonality: Polyclonal | Host: Goat | Reactivity: Mouse IgG1

10 % until end of 2023....

546 €