Blue Fluorescent Protein | BFP , clone 7F10

AS20 4486 | Clonality: Monoclonal | Host: Mouse | Reactivity: Blue Fluorescent Protein (BFP)

Product Information

Immunogen Recombinant EBFP (NCBI accession number AX_766758 REGION: 1-717, expression vector pGEX-1N), expressed in E.coli.

Host Mouse

Clonality Monoclonal

Subclass/isotype IgG1

Purity Immunoglobulin Protein A purified in a 10 mM ammonium bicarbonate buffer, with 2 mg of BSA.

Format Lyophilized

Quantity 100 µg

Reconstitution Recommended antibody concentration: 0.5 mg/ml (when dissolved at 0.5 mg/ml, the BSA concentration will be 1%).
Recommended solvent; 100 mM PBS or Tris-HCl, pH 7.0 •
Additional sodium azide ( up to 0.05%) is recommended for long term storage. •
For a 0.5 mg/ml antibody concentration in 1% BSA, dissolve in 200 μl buffer.

Storage Store lyophilized/reconstituted at 2-8°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA)

Recommended dilution 1 : 1000-1: 5000 (ELISA)

Reactivity

Confirmed reactivity BFP

Application examples

Application examples ELISA using anti-BFP antibodies

Specificity of Anti-Blue Fluorescent Protein (BFP) Immunoglobulin, clone 7F10, determined by ELISA. Antibody fraction (0.5 mg/ml) 900X diluted in PBS containing 0,05% tween-20 and 5% non fat dry milk. Antibody was tested on various recombinant protein substrates i.e. hRpp14-GST (pGEX-2T), hRrp46-HIS10 (pET16b), GST (pGEX-2T) and BSA, 98% (Sigma).

Additional information

Background

Background Fluorescent proteins, like BFP, can be used as protein "tags" to study the subcellular localization of proteins and/or their translocation upon stimulation and/or as markers for transfections in transient and stable expression systems.

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background:	Fluorescent proteins, like BFP, can be used as protein "tags" to study the subcellular localization of proteins and/or their translocation upon stimulation and/or as markers for transfections in transient and stable expression systems.
Picture (footer):	Specificity of Anti-Blue Fluorescent Protein (BFP) Immunoglobulin, clone 7F10, determined by ELISA. Antibody fraction (0.5 mg/ml) 900X diluted in PBS containing 0,05% tween-20 and 5% non fat dry milk. Antibody was tested on various recombinant protein substrates i.e. hRpp14-GST (pGEX-2T), hRrp46-HIS10 (pET16b), GST (pGEX-2T) and BSA, 98% (Sigma).
Confirmed reactivity:	BFP
Clonality:	Monoclonal
Format:	Lyophilized
Host:	Mouse
immunogen:	Recombinant EBFP (NCBI accession number AX_766758 REGION: 1-717, expression vector pGEX-1N), expressed in E.coli.
Purity:	Immunoglobulin Protein A purified in a 10 mM ammonium bicarbonate buffer, with 2 mg of BSA.
Quantity:	100 µg
recommended dilution:	1 : 1000-1: 5000 (ELISA)
Reconstitution:	Recommended antibody concentration: 0.5 mg/ml (when dissolved at 0.5 mg/ml, the BSA concentration will be 1%). Recommended solvent; 100 mM PBS or Tris-HCl, pH 7.0 • Additional sodium azide ( up to 0.05%) is recommended for long term storage. • For a 0.5 mg/ml antibody concentration in 1% BSA, dissolve in 200 μl buffer.
storage:	Store lyophilized/reconstituted at 2-8°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Sub class:	IgG1
tested applications:	ELISA (ELISA)