IKP

Anti-Blue Fluorescent Protein | BFP , clone 7F10

Product no: AS20 4486

AS20 4486 | Clonality: Monoclonal | Host: Mouse | Reactivity: Blue Fluorescent Protein (BFP)

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  • Product Info
  • Immunogen: Recombinant EBFP (NCBI accession number AX_766758 REGION: 1-717, expression vector pGEX-1N), expressed in E.coli.
    Sub class: IgG1
    Host: Mouse
    Clonality: Monoclonal
    Purity: Immunoglobulin Protein A purified in a 10 mM ammonium bicarbonate buffer, with 2 mg of BSA.
    Format: Lyophilized
    Quantity: 100 µg
    Reconstitution: Recommended antibody concentration: 0.5 mg/ml (when dissolved at 0.5 mg/ml, the BSA concentration will be 1%).
    Recommended solvent; 100 mM PBS or Tris-HCl, pH 7.0 •
    Additional sodium azide ( up to 0.05%) is recommended for long term storage. •
    For a 0.5 mg/ml antibody concentration in 1% BSA, dissolve in 200 μl buffer.
    Storage: Store lyophilized/reconstituted at 2-8°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA)
    Recommended dilution: 1 : 1000-1: 5000 (ELISA)
  • Reactivity
  • Confirmed reactivity: BFP
  • Application Examples
  • ELISA using anti-BFP antibodies

    Specificity of Anti-Blue Fluorescent Protein (BFP) Immunoglobulin, clone 7F10, determined by ELISA. Antibody fraction (0.5 mg/ml) 900X diluted in PBS containing 0,05% tween-20 and 5% non fat dry milk. Antibody was tested on various recombinant protein substrates i.e. hRpp14-GST (pGEX-2T), hRrp46-HIS10 (pET16b), GST (pGEX-2T) and BSA, 98% (Sigma).
  • Background
  • Background: Fluorescent proteins, like BFP, can be used as protein "tags" to study the subcellular localization of proteins and/or their translocation upon stimulation and/or as markers for transfections in transient and stable expression systems.
  • Protocols
  • Antibody protocols
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