Blue Fluorescent Protein | BFP , clone 7F10

AS20 4486 | Clonality: Monoclonal | Host: Mouse | Reactivity: Blue Fluorescent Protein (BFP)

Blue Fluorescent Protein | BFP , clone 7F10 in the group Tag Antibodies / GFP/EGFP/BFP/CFP/YFP/RFP at Agrisera AB (Antibodies for research) (AS20 4486)


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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen Recombinant EBFP (NCBI accession number AX_766758 REGION: 1-717, expression vector pGEX-1N), expressed in E.coli.
Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Purity Immunoglobulin Protein A purified in a 10 mM ammonium bicarbonate buffer, with 2 mg of BSA.
Format Lyophilized
Quantity 100 ĩg
Reconstitution Recommended antibody concentration: 0.5 mg/ml (when dissolved at 0.5 mg/ml, the BSA concentration will be 1%).
Recommended solvent; 100 mM PBS or Tris-HCl, pH 7.0 •
Additional sodium azide ( up to 0.05%) is recommended for long term storage. •
For a 0.5 mg/ml antibody concentration in 1% BSA, dissolve in 200 μl buffer.
Storage Store lyophilized/reconstituted at 2-8°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications ELISA (ELISA)
Recommended dilution 1 : 1000-1: 5000 (ELISA)


Confirmed reactivity BFP

Application examples

Application examples ELISA using anti-BFP antibodies

Specificity of Anti-Blue Fluorescent Protein (BFP) Immunoglobulin, clone 7F10, determined by ELISA. Antibody fraction (0.5 mg/ml) 900X diluted in PBS containing 0,05% tween-20 and 5% non fat dry milk. Antibody was tested on various recombinant protein substrates i.e. hRpp14-GST (pGEX-2T), hRrp46-HIS10 (pET16b), GST (pGEX-2T) and BSA, 98% (Sigma).

Additional information

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Background Fluorescent proteins, like BFP, can be used as protein "tags" to study the subcellular localization of proteins and/or their translocation upon stimulation and/or as markers for transfections in transient and stable expression systems.

Product citations

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