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Blue Fluorescent Protein | BFP , clone 7F10

AS20 4486 | Clonality: Monoclonal | Host: Mouse | Reactivity: Blue Fluorescent Protein (BFP)

Blue Fluorescent Protein | BFP , clone 7F10 in the group Tag Antibodies / GFP/YFP/RFP/CFP at Agrisera AB (Antibodies for research) (AS20 4486)

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Product name, number (Agrisera, Sweden)

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Product Information

Immunogen Recombinant EBFP (NCBI accession number AX_766758 REGION: 1-717, expression vector pGEX-1N), expressed in E.coli.
Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Purity Purified on Protein A
Format

Lyophilized in a 10 mM ammonium bicarbonate buffer. Each vial contains 2 mg of BSA.

Quantity 100 µg
Reconstitution Recommended antibody concentration: 0.5 mg/ml (when dissolved at 0.5 mg/ml, the BSA concentration will be 1%).
Recommended solvent; 100 mM PBS or Tris-HCl, pH 7.0 •
Additional sodium azide ( up to 0.05%) is recommended for long term storage. •
For a 0.5 mg/ml antibody concentration in 1% BSA, dissolve in 200 μl buffer.
Storage Store lyophilized/reconstituted at 2-8°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA)
Recommended dilution 1 : 1000-1: 5000 (ELISA)

Reactivity

Confirmed reactivity BFP

Application examples

Application examples ELISA using anti-BFP antibodies

Specificity of Anti-Blue Fluorescent Protein (BFP) Immunoglobulin, clone 7F10, determined by ELISA. Antibody fraction (0.5 mg/ml) 900X diluted in PBS containing 0,05% tween-20 and 5% non fat dry milk. Antibody was tested on various recombinant protein substrates i.e. hRpp14-GST (pGEX-2T), hRrp46-HIS10 (pET16b), GST (pGEX-2T) and BSA, 98% (Sigma).

Additional information

Related products

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AS15 2999 | Anti-GFP (total IgG), rabbit antibody
AS15 3001 | Anti-GFP, peroxidase conjugated, rabbit antibody
AS15 3002 | GFP (purified recombinant protein) Other GFP-tag antibodies.
Other tag antibodies.

Background

Background Fluorescent proteins, like BFP, can be used as protein "tags" to study the subcellular localization of proteins and/or their translocation upon stimulation and/or as markers for transfections in transient and stable expression systems.

Product citations

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