Blue Fluorescent Protein | BFP , clone 7F10
AS20 4486 | Clonality: Monoclonal | Host: Mouse | Reactivity: Blue Fluorescent Protein (BFP)

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Product Information
Immunogen
Recombinant EBFP (NCBI accession number AX_766758 REGION: 1-717, expression vector pGEX-1N), expressed in E.coli.
Host
Mouse
Clonality
Monoclonal
Subclass/isotype
IgG1
Purity
Immunoglobulin Protein A purified in a 10 mM ammonium bicarbonate buffer, with 2 mg of BSA.
Format
Lyophilized
Quantity
100 µg
Reconstitution
Recommended antibody concentration: 0.5 mg/ml (when dissolved at 0.5 mg/ml, the BSA concentration will be 1%).
Recommended solvent; 100 mM PBS or Tris-HCl, pH 7.0 •
Additional sodium azide ( up to 0.05%) is recommended for long term storage. •
For a 0.5 mg/ml antibody concentration in 1% BSA, dissolve in 200 μl buffer.
Recommended solvent; 100 mM PBS or Tris-HCl, pH 7.0 •
Additional sodium azide ( up to 0.05%) is recommended for long term storage. •
For a 0.5 mg/ml antibody concentration in 1% BSA, dissolve in 200 μl buffer.
Storage
Store lyophilized/reconstituted at 2-8°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
ELISA (ELISA)
Recommended dilution
1 : 1000-1: 5000 (ELISA)
Reactivity
Confirmed reactivity
BFP
Application examples
Application examples
Specificity of Anti-Blue Fluorescent Protein (BFP) Immunoglobulin, clone 7F10, determined by ELISA. Antibody fraction (0.5 mg/ml) 900X diluted in PBS containing 0,05% tween-20 and 5% non fat dry milk. Antibody was tested on various recombinant protein substrates i.e. hRpp14-GST (pGEX-2T), hRrp46-HIS10 (pET16b), GST (pGEX-2T) and BSA, 98% (Sigma).

Specificity of Anti-Blue Fluorescent Protein (BFP) Immunoglobulin, clone 7F10, determined by ELISA. Antibody fraction (0.5 mg/ml) 900X diluted in PBS containing 0,05% tween-20 and 5% non fat dry milk. Antibody was tested on various recombinant protein substrates i.e. hRpp14-GST (pGEX-2T), hRrp46-HIS10 (pET16b), GST (pGEX-2T) and BSA, 98% (Sigma).
Additional information
Background
Background
Fluorescent proteins, like BFP, can be used as protein "tags" to study the subcellular localization of proteins and/or their translocation upon stimulation and/or as markers for transfections in transient and stable expression systems.
Product citations
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