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GUS | Beta-glucuronidase

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AS16 3689
| Clonality: Polyclonal | Host: Rabbit | Reactivity: GUS from Escherichia coli

GUS | Beta-glucuronidase in the group Tag Antibodies / GAL4 / GUS / LUC / others at Agrisera AB (Antibodies for research) (AS16 3689)

DATA SHEET IN PDF

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How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Escherichia coli GUS protein at amino acid 358.

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 10 000 (WB)
Expected | apparent MW

depends upon a MW of a protein which is expressed with GUS

Reactivity

Confirmed reactivity GUS
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application example

western blot using anti-GUS antibodies 

100 µg of total protein from Arabidopsis thaliana wild-type (1) and transgenic plants expressing GUS under HRT promoter (2) were extracted with write buffer containing  50 mM Tris-HCl pH 7.5, 150 mM NaCl, 10 mM MgCl2, 5 mM EDTA, 10% glycerol, 0.1% NP-40, 0.1% protease inhibitor cocktail . The proteins were denatured by boiling for 5 minwere separated on 10% SDS-PAGE  and blotted 30 min to PVDF using tank transfer. Blots were blocked with  TBST containing 5% non-fat dry milk   for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 for 2h at RT with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 15 min each in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in  for 1h at RT with agitation. The blot was washed as above and developed with  Clarity MAX (Bio-RAD). Exposure time was 10 seconds.

Courtesy of Dr. Pradeep Kachroo, University of Kentucky, USA

Additional information

Additional information This antibody will not work with pCAMBIA vectors due to mutation starting at amino acid position 358.

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Background

Background GUS (Beta-glucuronidase) is an enzyme which when incubated with colorless or non-fluorescent substrates, can catalyze reaction of their transformation into coloured or fluorescent products.

Product citations

Selected references Nizkorodova et al. (2020). The Effect of Translation Promoting Site (TPS) on Protein Expression in E. coli Cells. Mol Biotechnol (2020). doi.org/10.1007/s12033-020-00251-1
Nikorodova and Isakov (2019). New insights into the mechanism of action of e-element enhancer in E.coli. Eurasian Journal of Applied Biotechnology 2/2019

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