GUS | Beta-glucuronidase

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AS16 3689 | Clonality: Polyclonal | Host: Rabbit | Reactivity: GUS from Escherichia coli

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product information
Background		GUS (Beta-glucuronidase) is an enzyme which when incubated with colorless or non-fluorescent substrates, can catalyze reaction of their transformation into coloured or fluorescent products.
Immunogen		KLH-conjugated synthetic peptide derived from Escherichia coli GUS protein at amino acid 358.
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Serum
Format		Lyophilized
Quantity		50 µl
Reconstitution		For reconstitution add 50 µl of sterile water.
Storage		Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications		Western blot (WB)
Related products		Antibodies to other reporter proteins Secondary antibodies
Additional information		This antibody will not work with pCAMBIA vectors due to mutation starting at amino acid position 358.

application information
Recommended dilution		1 : 10 000 (WB)
Expected \| apparent MW		depends upon a MW of a protein which is expressed with GUS
Confirmed reactivity		GUS
Predicted reactivity
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references		To be added when available, antibody released in July 2017.

Application example

western blot using anti-GUS antibodies

100 µg of total protein from Arabidopsis thaliana wild-type (1) and transgenic plants expressing GUS under HRT promoter (2) were extracted with write buffer containing 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 10 mM MgCl₂, 5 mM EDTA, 10% glycerol, 0.1% NP-40, 0.1% protease inhibitor cocktail . The proteins were denatured by boiling for 5 minwere separated on 10% SDS-PAGE and blotted 30 min to PVDF using tank transfer. Blots were blocked with TBST containing 5% non-fat dry milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 for 2h at RT with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 15 min each in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed with Clarity MAX (Bio-RAD). Exposure time was 10 seconds.

Courtesy of Dr. Pradeep Kachroo, University of Kentucky, USA

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