Lhcb4 | CP29 chlorophyll a/b binding protein of plant PSII

AS04 045  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana, C. sativus, H. vulgare, N. tabacum, O. sativa, P. sativum, P. vulgaris, T. aestivum, Triticale, Z. mays

Lhcb4 | CP29 chlorophyll a/b binding protein of plant PSII  in the group Antibodies for Plant/Algal  / Photosynthesis  / LHC at Agrisera AB (Antibodies for research) (AS04 045)


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Product Information


BSA-conjugated synthetic peptide derived from a highly conserved sequence of Lhb4 proteins from angiosperms (monocots and dicots) and gymnosperms, including Arabidopsis thaliana (Lhcb4.1 At5g01530 and Lhcb4.2 At3g08940 and Lhcb4.3 At2G40100).

Host Rabbit
Clonality Polyclonal
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 0.5 mg
Reconstitution For reconstitution add 250 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 7 000 (WB)
Expected | apparent MW

31.9 | 29 kDa for Arabidopsis thaliana


Confirmed reactivity Arabidopsis thaliana, Cucumis sativus L. cv. Jihong no. 2, Drosera capensis, Hordeum vulgare, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Triticum aestivum, Triticale, Zea mays
Predicted reactivity

Catalpa bungei, Cucumis sativus, Populus, gymnosperms and microalgae Ostrecococcus tauri; the target sequence is only weakly conserved in Physcomitrella patens

Species of your interest not listed? Contact us
Not reactive in

Chlamydomonas reinhardtii (please use AS06 117 for this organism)

Application examples

Application examples Application example

Western blot using anti-Lhcb4 antibodies

5 µg of total protein from embebed seeds of Nicotiana tabacum growing during 4 d in dark (0) and then transfer to continue light growing for 6 h (6) extracted with LB2x buffer and denatured 90 ºC for 2-5 min, were separated on 12.5 % SDS-PAGE and blotted 1h to PVDF using tank transfer. Blots were blocked with TBS-T with 5% dry-milk for 3h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 overnight at 4 ºC with agitation in TBS-T with 5% dry-milk. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4 times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602, from Agrisera) diluted to 1:30 000 in TBS-T with 5% dry-milk for 1h at RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescent detection. Exposure time was 60 seconds.

Courtesy of Dr. Concha Almoguera, Inst. de Recursos Naturales y Agrobiología –CSIC, Spain

Additional information

Additional information

An overview about the different Lhc-protein types in plants can be found in Klimmek et al. (2006) Abundantly and rarely expressed Lhc protein genes exhibit distinct regulation patterns in plants. Plant Physiol 140: 793-804.

Lhcb4 protein is processed into mature form (Jansson 1999).

Related products

Related products

AS06 117 | Anti-Lhcb4 | CP29 (Lhcb4) homolog, (Chlamydomonas), rabbit antibodies

LHC antibodies against pigment-binding proteins

PSII  antibodies against Photosystem II proteins

AS04 045PRE Lhcb4 | CP29 chlorophyll a/b binding protein of plant PSII, pre-immune serum

Plant protein extraction buffer

Secondary antibodies



Lhcb4 (CP29) is one of the 3 minor chlrorophyll a/b-binding proteins associated with Photosystem II in plants and algae. Lhcb4 has been suggested to act in the regulation of the chl a excited state concentration of PSII because of its ability of sensing lumenal pH resulting in reversible phosphorylation. In Arabidopsis thaliana 2 genes code for two isoforms Lhcb4.1 and Lhcb4.2. A third isoform (Lhcb4.3, At2g40100), probably only present in dicots, has found to be differently regulated and therefore has been denoted as Lhcb8.

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