Lhcb4 | CP29 chlorophyll a/b binding protein of plant PSII
AS04 045 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, C. sativus, H. vulgare, N. tabacum, O. sativa, P. sativum, P. vulgaris, T. aestivum, Triticale, Z. mays
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Product Information
BSA-conjugated synthetic peptide derived from a highly conserved sequence of Lhb4 proteins from angiosperms (monocots and dicots) and gymnosperms, including Arabidopsis thaliana (Lhcb4.1 At5g01530 and Lhcb4.2 At3g08940 and Lhcb4.3 At2G40100).
31.9 | 29 kDa for Arabidopsis thaliana
Reactivity
Catalpa bungei, Cucumis sativus, Populus, gymnosperms and microalgae Ostrecococcus tauri; the target sequence is only weakly conserved in Physcomitrella patens
Chlamydomonas reinhardtii (please use AS06 117 for this organism)
Application examples
5 µg of total protein from embebed seeds of Nicotiana tabacum growing during 4 d in dark (0) and then transfer to continue light growing for 6 h (6) extracted with LB2x buffer and denatured 90 ºC for 2-5 min, were separated on 12.5 % SDS-PAGE and blotted 1h to PVDF using tank transfer. Blots were blocked with TBS-T with 5% dry-milk for 3h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 overnight at 4 ºC with agitation in TBS-T with 5% dry-milk. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4 times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602, from Agrisera) diluted to 1:30 000 in TBS-T with 5% dry-milk for 1h at RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescent detection. Exposure time was 60 seconds.
Courtesy of Dr. Concha Almoguera, Inst. de Recursos Naturales y Agrobiología –CSIC, Spain
Additional information
An overview about the different Lhc-protein types in plants can be found in Klimmek et al. (2006) Abundantly and rarely expressed Lhc protein genes exhibit distinct regulation patterns in plants. Plant Physiol 140: 793-804.
Lhcb4 protein is processed into mature form (Jansson 1999).
Background
Lhcb4 (CP29) is one of the 3 minor chlrorophyll a/b-binding proteins associated with Photosystem II in plants and algae. Lhcb4 has been suggested to act in the regulation of the chl a excited state concentration of PSII because of its ability of sensing lumenal pH resulting in reversible phosphorylation. In Arabidopsis thaliana 2 genes code for two isoforms Lhcb4.1 and Lhcb4.2. A third isoform (Lhcb4.3, At2g40100), probably only present in dicots, has found to be differently regulated and therefore has been denoted as Lhcb8.
Product citations
Rogowski et al. (2019). Photosynthesis and organization of maize mesophyll and bundle sheath thylakoids of plants grown in various light intensities. Environmental and Experimental Botany Volume 162, June 2019, Pages 72-86.
Mao et al. (2018). Comparison on Photosynthesis and Antioxidant Defense Systems in Wheat with Different Ploidy Levels and Octoploid Triticale. Int J Mol Sci. 2018 Oct 2;19(10). pii: E3006. doi: 10.3390/ijms19103006.
Geem at al. (2018). Jasmonic acid-inducible TSA1 facilitates ER body formation. Plant J. 2018 Sep 28. doi: 10.1111/tpj.14112.
Myouga et al. (2018). Stable accumulation of photosystem II requires ONE-HELIX PROTEIN1 (OHP1) of the light harvesting-like family. Plant Physiol. 2018 Feb 1. pii: pp.01782.2017. doi: 10.1104/pp.17.01782.
Kim et al. (2018). The rice zebra3 (z3) mutation disrupts citrate distribution and produces transverse dark-green/green variegation in mature leaves. Rice (N Y). 2018 Jan 5;11(1):1. doi: 10.1186/s12284-017-0196-8.
Tyuereva et al. (2017). The absence of chlorophyll b affects lateral mobility of photosynthetic complexes and lipids in grana membranes of Arabidopsis and barley chlorina mutants. Photosynth Res. 2017 Apr 5. doi: 10.1007/s11120-017-0376-9. (Hordeum vulgare, western blot)
Yang-Er Chen et al. (2017). Responses of photosystem II and antioxidative systems to high light and high temperature co-stress in wheat. J. of Exp. Botany, Volume 135, March 2017, Pages 45–55.
Betterle et al. (2016). The STN8 kinase-PBCP phosphatase system is responsible for high-light-induced reversible phosphorylation of the PSII inner antenna subunit CP29 in rice. Plant J. 2016 Nov 4. doi: 10.1111/tpj.13412. [Epub ahead of print]
Pavlovič et al. (2016). A carnivorous sundew plant prefers protein over chitin as a source of nitrogen from its traps. Plant Physiol Biochem. 2016 Mar 5;104:11-16. doi: 10.1016/j.plaphy.2016.03.008
Kim et al. (2015). Cytosolic targeting factor AKR2A captures chloroplast outer membrane-localized client proteins at the ribosome during translation. Nat Commun. 2015 Apr 16;6:6843. doi: 10.1038/ncomms7843.
Sun et al. (2014). Direct energy transfer from the major antenna to the photosystem II core complexes in the absence of minor antennae in liposomes. Biochim Biophys Acta. 2014 Nov 22. pii: S0005-2728(14)00650-1. doi: 10.1016/j.bbabio.2014.11.005.
Grimmer et al. (2014). The RNA-binding protein RNP29 is an unusual Toc159 transport substrate. Front. Plant Sci. | doi: 10.3389/fpls.2014.00258
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