Lhcb2-P | LHCII type II chlorophyll a/b-binding protein, phosphorylated
AS13 2705 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana, Echinochloa crus-galli, Zea mays
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KLH-conjugated synthetic peptide: RRT*VKSTPQS, where T* indicates phospho-Thr
25 | 25 kDa for Arabidopsis thaliana
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1 µg of thylakoid membranes isolated from Arabidopsis thaliana wild-type and respective mutants were solubilized with 3X LB (6 M urea, 12% SDS, 30% glycerol, 100 mM DTT, 150 mM Tris pH7.0, 0.8% Comassie G-250). 1 µg of total chlorophyll was loaded and separated on 16% SDS-PAGE, and then blotted for 2 h onto nitrocellulose membrane. Blots were blocked with milk powder for 2 h and then incubated in the primary antibody solution (AS13 2705, 1: 5 000) for 2.5 h, which was then decanted and the blot was washed 3 times for 5 min in TBST. Membrane was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1 h, followed by washing steps as above. All the steps fallowing transfer were performed in room temperature (RT) with agitation. Membrane was developed for 5 min with ECL according to the manufacturer’s instructions and recorded using FujiFilm CCD camera with 30 s increment time for around 5 min.
Courtesy of a phd candidate Małgorzata Pietrzykowska, Umeå Plant Science Centre, Sweden
Courtesy Dr. Wiola Wasilewska, Faculty of Biology, University of Warsaw, Poland
The major light-harvesting antenna complex II (LHCII) in photosynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. Lhcb2 is often coded by several nuclear genes and is found together with Lhcb1 within the mobile LHCII trimers involved in state1-state2 transition.
Vietoshkina et al. (2019). Comparison of State Transitions of the Photosynthetic Antennae in Arabidopsis and Barley Plants upon Illumination with Light of Various Intensity. Biochemistry (Moscow), Vol 84, Issue 9, pp 1065Ã¢??1073
Bychkov et al. (2019). Melatonin modifies the expression of the genes for nuclear- and plastid-encoded chloroplast proteins in detached Arabidopsis leaves exposed to photooxidative stress. Plant Physiology and Biochemistry, doi.org/10.1016/j.plaphy.2019.10.013.
Gasulla et al. (2018). Chlororespiration induces non-photochemical quenching of chlorophyll fluorescence during darkness in lichen chlorobionts. Physiol Plant. 2018 Jun 27. doi: 10.1111/ppl.12792.
Rantala and Tikkanen et al. (2018). Phosphorylation‐induced lateral rearrangements of thylakoid protein complexes upon light acclimation. Plant Direct Vol. 2, Issue 2.
Rantala et al. (2017). Proteomic characterization of hierarchical megacomplex formation in Arabidopsis thylakoid membrane. Plant J. 2017 Dec;92(5):951-962. doi: 10.1111/tpj.13732.
Fristedt et al. (2017). PSB33 sustains photosystem II D1 protein under fluctuating light conditions. Journal of Experimental Botany doi:10.1093/jxb/erx218.
Sato et al. (2015). Chlorophyll b degradation by chlorophyll b reductase under high-light conditions. Photosynth Res. 2015 Apr 21.
Leoni et al. (2013). Very rapid phosphorylation kinetics suggest a unique role for Lhcb2 during state transitions in Arabidopsis. Plant J. Oct;76(2):236-46. doi: 10.1111/tpj.12297. Epub 2013 Aug 26.
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