Q: I purchased an antibody from Agrisera and need some technichal guidance. What should I do?

 A: Contact Agrisera Technical Support for help. We will promptly reply to your questions. To be able provide you with the best help, we will need the following information: 

- Image of the obtained results (whole membrane with MW markers for Western blot, section image for immunolocalization)

- Exact details of the applied protocol

Once we receive the results with all experimental details included, we will provide suggestions on how to solve the issue and/or optimize the protocol.

Each case is solved individually, as there can be various reasons as to why a desired result cannot be obtained.

You will receive prompt and professional support. 
Contact us for help!



 Most common reasons why the desired result can not be obtained with a given antibody:

Wrong antibody was purchased. Sequence used to elicit an antibody, is not found in the protein which is to be visualized.

The sample contains too little of the target protein. For proteins of low abundance, the specific cellular fraction needs to be analyzed.

The sample was not harvested in conditions, in which the target protein is most abundant in the tissue, or the extraction buffer was not optimal (for example: no specific protease or proteasome inhibitors were included). 

Antibody was incubated for too short time. Always check the product information sheet for guidance, or contact the supplier. 

 






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