Antibody validation strategies, interview with experts

With millions of catalog antibodies available, the antibody product datasheets should be carefully analyzed, in terms of specific validation data, before choosing an antibody for your experimental setup.

Poor antibody validation contributes substantially to the ongoing reproducibility crisis. There is a lack of universal guidelines in antibody validation, as well as in how antibodies should be cited in scientific publications.

In a recent Editorial Article published by Biocompare, tilted: "Advanced Antibody Validation Strategies to Ensure Scientific Reproducibility" many important aspects of the antibody validation process were covered, and discussed by experts in the field. The interviewees were:

Dr. Joanna Porankiewicz-Asplund, Customer Support Manager at Agrisera Antibodies
Shamim Mohammad, Ph.D., Lead Scientist at ATCC
Tracey Long Ph.D., Senior Marketing Manager, Catalog Antibodies, Life Science Group at Bio-Rad Laboratories, Inc
Julia Gilden, Ph.D., Sr. Research Scientist Promega

Article excerpt to pay specific attention to:

Another common validation strategy involves Western blot detection of the recombinant protein. However, Porankiewicz Asplund cautions that this can be misleading if the antibody datasheet is not read carefully. "Based on this type of data, researchers might expect to see a very intense band on a Western blot, not realizing that it is impossible to achieve this in an endogenous extract, for a target of low abundance," she says. Protein abundance can be easily checked in PaxDb.

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Antibody validation strategies, interview with experts

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