Goat anti-Rabbit IgG (H&L), DyLight® 550 conjugated

AS11 1814 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

Product Information

Immunogen

Purified Rabbit IgG, whole molecule

Host Goat

Clonality Polyclonal

Purity Immunogen affinity purified goat IgG.

Format Lyophilized

Quantity 1 mg

Reconstitution For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Tested applications Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunofluorescence (IF)

Recommended dilution 1 : 50- 1 : 5 000 (ICC), 1 : 20- 1 : 2000 (IHC), 1 : 3000 (IF)

Reactivity

Confirmed reactivity Rabbit IgG heavy and light chains (H&L)

Predicted reactivity Rabbit IgG Heavy and Light chains (H&L)

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

DyLight® 550 (Ex = 550 nm; Em = 576 nm)

Based in immunoelectrophoresis, this antibody reacts with heavy chains on rabbit IgG and light chains on all rabbit immunoglobulins.

No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis.

Purity of this antibody is > 95% based on SDS-PAGE.

Background

Goat anti-rabbit IgG, DyLight® 550 Conjugate is a secondary antibody conjugated to
DyLight® 550, which binds to all rabbit IgGs in immunological assays.

DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Product citations

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