Goat anti-Rabbit IgG (H&L), DyLight® 350 conjugated

Name: Goat anti-Rabbit IgG (H&L), DyLight® 350 conjugated
SKU: AS11 1812
Price: 148 EUR
Availability: InStock

AS11 1812 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

Goat anti-Rabbit IgG (H&L), DyLight® 350 conjugated in the group Secondary Antibodies / Anti-Rabbit / Fluorescent / DyLight® / DyLight® 350 at Agrisera AB (Antibodies for research) (AS11 1812)

Product Information

Immunogen

Purified Rabbit IgG, whole molecule

Host Goat

Clonality Polyclonal

Purity Immunogen affinity purified goat IgG.

Format Lyophilized

Quantity 1 mg

Reconstitution For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

Storage Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Tested applications Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunofluorescence (IF)

Recommended dilution 1 : 50- 1 : 5 000 (ICC), 1 : 20- 1 : 2000 (IHC), 1 : 3000 (IF)

Reactivity

Confirmed reactivity Rabbit IgG heavy and light chains (H&L)

Predicted reactivity Rabbit IgG Heavy and Light chains (H&L)

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Conjugate is present in 10 mM Sodium Phosphate,
0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free.
0.05 % (w/v) sodium azide is added as preservative.

DyLight® 350 has a maximum absorbance at 353 nm; Emax = 432 nm.

Based in immunoelectrophoresis, this antibody reacts with heavy chains on rabbit IgG and light chains on all rabbit immunoglobulins.

No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis.

Purity of this antibody is > 95% based on SDS-PAGE.

Background

Goat anti-rabbit IgG, DyLight® 350 Conjugate is a secondary antibody conjugated to
DyLight® 350, which binds to all rabbit IgGs in immunological assays.

DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Product citations

immunogen:	Purified Rabbit IgG, whole molecule
Host:	Goat
Clonality:	Polyclonal
Purity:	Immunogen affinity purified goat IgG.
Format:	Lyophilized
Quantity:	1 mg
Reconstitution:	For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
storage:	Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications:	Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunofluorescence (IF)
recommended dilution:	1 : 50- 1 : 5 000 (ICC), 1 : 20- 1 : 2000 (IHC), 1 : 3000 (IF)
Confirmed reactivity:	Rabbit IgG heavy and light chains (H&L)
predicted reactivity:	Rabbit IgG Heavy and Light chains (H&L)
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative. DyLight® 350 has a maximum absorbance at 353 nm; Emax = 432 nm.
additional information (application):	Based in immunoelectrophoresis, this antibody reacts with heavy chains on rabbit IgG and light chains on all rabbit immunoglobulins. No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis. Purity of this antibody is > 95% based on SDS-PAGE.
background:	Goat anti-rabbit IgG, DyLight® 350 Conjugate is a secondary antibody conjugated to DyLight® 350, which binds to all rabbit IgGs in immunological assays. DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

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