Goat anti-Rabbit IgG (H&L), DyLight® 633 conjugated

Product no: AS11 1816

AS11 1816 | Clonality: Polyclonal  |  Host: Goat  | Reactivity: Rabbit IgG (H&L)

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  • Product Info
  • Immunogen:

    Purified Rabbit IgG, whole molecule

    Host: Goat
    Clonality: Polyclonal
    Purity: Immunogen affinity purified goat IgG.
    Format: Lyophilized
    Quantity: 1 mg
    Reconstitution: For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
    Storage: Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
    Tested applications: Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunofluorescence (IF)
    Recommended dilution: 1 : 50- 1 : 5 000 (ICC), 1 : 20- 1 : 2000 (IHC), 1 : 3000 (IF)
  • Reactivity
  • Confirmed reactivity: Rabbit IgG heavy and light chains (H&L)
    Predicted reactivity: Rabbit IgG Heavy and Light chains (H&L)
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information:

    Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.

    DyLight® 633  (Ex = 638 nm; Em = 658 nm)

    Additional information (application):

    Based in immunoelectrophoresis, this antibody reacts with heavy chains on rabbit IgG and light chains on all rabbit immunoglobulins.

    No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis. 

    Purity of this antibody is > 95% based on SDS-PAGE.

  • Background
  • Background:

    Goat anti-rabbit IgG, DyLight® 633 Conjugate is a secondary antibody conjugated to  DyLight® 633, which binds to all rabbit IgGs in immunological assays.

    DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

  • Product Citations
  • Selected references: 38221900
  • Protocols
  • Antibody protocols
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