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PsaE | PSI-E subunit of photosystem I (cyanobacterial)

AS19 4355  |  Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Synechocystis sp. PCC 6803, Thermosynechococcus elongatus

PsaE | PSI-E subunit of photosystem I (cyanobacterial) in the group Antibodies for Plant/Algal  / Cyanobacteria at Agrisera AB (Antibodies for research) (AS19 4355)

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Product Information

Immunogen Recombinant, full length PsaE of Thermosynechococcus elongatus, overexpressed in E.coli, UniProt: P0A423
Host Rabbit
Clonality Polyclonal
Purity Serum
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 5000 - 1: 10 000 (WB)
Expected | apparent MW

8 kDa

Reactivity

Confirmed reactivity Synechocystis sp. PCC 6803, Thermosynechococcus elongatus
Predicted reactivity Cyanidioschyzon merolae (strain 10D), Synechococcus elongatus PCC 6301, Synechococcus sp. PCC 7002, Trichodesmium erythraeum, Trichormus variabilis

Application examples

Application examples Western blot using anti-cyanobacterial PsaE antibodies


Soluble (8 μg protein) and membrane proteins (corresponding to 1 μg of chlorophyll a) from Synechocystis sp. PCC 6803
1 – WT (soluble)
2 - DPsaE – PsaE deletion (soluble)
3 - EY10 – PsaE-HoxY fusion (10 aa linker) (soluble)
4 - EY16 – PsaE-HoxY fusion (16 aa linker) (soluble)
5 - EU – PsaE-HoxU fusion (soluble)
6 – WT (membrane)
7 - DPsaE – PsaE deletion (membrane)
were extracted with and resuspended in ACA buffer (750 mM e- amino caproic acid; 50 mM BisTris/HCl, pH 7.0; 0.5 mM EDTA). Samples were denatured with 2x sample buffer (125 mM Tris, pH=6,8; 200 mM DTT; 4% (w/v) SDS; 20% (w/v) Glycerin; 0,02% (w/v) bromophenol blue) at room temperature (RT) for 1h. The proteins were separated on 17.5 % SDS PAGE (Bis-Tris) gels and blotted for 60 min onto a nitrocellulose membrane using a wet transfer system (BioRad). The membrane was blocked with 5% milk powder in PBS-T for 1 h at RT with agitation. The blot was then incubated overnight with the primary antibody at a dilution of 1:5.000 in PBS-T at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed three times for 20 min in PBS-T with agitation. The blot was incubated using a matching secondary antibody (anti-rabbit IgG horseradish peroxidase conjugated) diluted to 1:10.000 in PBS-T for 1 h at RT with agitation. The blot was washed three times for 10 min with PBS-T and two times for 10 minutes with PBS. Subsequently the membrane was incubated with chemiluminenscent detection reagent. The differently exposed films were developed using a NDT DÜRR developer.

Courtesy of Dr.Marko Boehm,Botanisches Institut der Christian-Albrechts-Universität zu Kiel, Germany

Additional information

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Background

Background PsaE is a nucleus encoded subunit of the Photosystem I reaction center. It is located on the stroma side and interacts with PsaF. PsaE may be involved in Fd reduction. Alternative names:Photosystem I reaction center subunit IV, Photosystem I 8.1 kDa protein, p30 protein

Product citations

Selected references To be added when available, antibody released in November 2020.

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