PsaE | PSI-E subunit of photosystem I (cyanobacterial)
AS19 4355 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Synechocystis sp. PCC 6803, Thermosynechococcus elongatus
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Product Information
Immunogen
Recombinant, full length PsaE of Thermosynechococcus elongatus, overexpressed in E.coli, UniProt: P0A423
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Quantity
50 µl
Reconstitution
For reconstitution add 50 µl of sterile water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Western blot (WB)
Recommended dilution
1 : 5000 - 1: 10 000 (WB)
Expected | apparent MW
8 kDa
Reactivity
Confirmed reactivity
Synechocystis sp. PCC 6803, Thermosynechococcus elongatus
Predicted reactivity
Cyanidioschyzon merolae (strain 10D), Synechococcus elongatus PCC 6301, Synechococcus sp. PCC 7002, Trichodesmium erythraeum, Trichormus variabilis
Application examples
Application examples
Soluble (8 μg protein) and membrane proteins (corresponding to 1 μg of chlorophyll a) from Synechocystis sp. PCC 6803
1 – WT (soluble)
2 - DPsaE – PsaE deletion (soluble)
3 - EY10 – PsaE-HoxY fusion (10 aa linker) (soluble)
4 - EY16 – PsaE-HoxY fusion (16 aa linker) (soluble)
5 - EU – PsaE-HoxU fusion (soluble)
6 – WT (membrane)
7 - DPsaE – PsaE deletion (membrane)
were extracted with and resuspended in ACA buffer (750 mM e- amino caproic acid; 50 mM BisTris/HCl, pH 7.0; 0.5 mM EDTA). Samples were denatured with 2x sample buffer (125 mM Tris, pH=6,8; 200 mM DTT; 4% (w/v) SDS; 20% (w/v) Glycerin; 0,02% (w/v) bromophenol blue) at room temperature (RT) for 1h. The proteins were separated on 17.5 % SDS PAGE (Bis-Tris) gels and blotted for 60 min onto a nitrocellulose membrane using a wet transfer system (BioRad). The membrane was blocked with 5% milk powder in PBS-T for 1 h at RT with agitation. The blot was then incubated overnight with the primary antibody at a dilution of 1:5.000 in PBS-T at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed three times for 20 min in PBS-T with agitation. The blot was incubated using a matching secondary antibody (anti-rabbit IgG horseradish peroxidase conjugated) diluted to 1:10.000 in PBS-T for 1 h at RT with agitation. The blot was washed three times for 10 min with PBS-T and two times for 10 minutes with PBS. Subsequently the membrane was incubated with chemiluminenscent detection reagent. The differently exposed films were developed using a NDT DÜRR developer.
Soluble (8 μg protein) and membrane proteins (corresponding to 1 μg of chlorophyll a) from Synechocystis sp. PCC 6803
1 – WT (soluble)
2 - DPsaE – PsaE deletion (soluble)
3 - EY10 – PsaE-HoxY fusion (10 aa linker) (soluble)
4 - EY16 – PsaE-HoxY fusion (16 aa linker) (soluble)
5 - EU – PsaE-HoxU fusion (soluble)
6 – WT (membrane)
7 - DPsaE – PsaE deletion (membrane)
were extracted with and resuspended in ACA buffer (750 mM e- amino caproic acid; 50 mM BisTris/HCl, pH 7.0; 0.5 mM EDTA). Samples were denatured with 2x sample buffer (125 mM Tris, pH=6,8; 200 mM DTT; 4% (w/v) SDS; 20% (w/v) Glycerin; 0,02% (w/v) bromophenol blue) at room temperature (RT) for 1h. The proteins were separated on 17.5 % SDS PAGE (Bis-Tris) gels and blotted for 60 min onto a nitrocellulose membrane using a wet transfer system (BioRad). The membrane was blocked with 5% milk powder in PBS-T for 1 h at RT with agitation. The blot was then incubated overnight with the primary antibody at a dilution of 1:5.000 in PBS-T at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed three times for 20 min in PBS-T with agitation. The blot was incubated using a matching secondary antibody (anti-rabbit IgG horseradish peroxidase conjugated) diluted to 1:10.000 in PBS-T for 1 h at RT with agitation. The blot was washed three times for 10 min with PBS-T and two times for 10 minutes with PBS. Subsequently the membrane was incubated with chemiluminenscent detection reagent. The differently exposed films were developed using a NDT DÜRR developer.
Courtesy of Dr.Marko Boehm,Botanisches Institut der Christian-Albrechts-Universität zu Kiel, Germany
Additional information
Background
Background
PsaE is a nucleus encoded subunit of the Photosystem I reaction center. It is located on the stroma side and interacts with PsaF. PsaE may be involved in Fd reduction. Alternative names:Photosystem I reaction center subunit IV, Photosystem I 8.1 kDa protein, p30 protein
Product citations
Selected references
To be added when available, antibody released in November 2020.
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