PsaF | PSI-F subunit of photosystem I (cyanobacterial)

AS19 4356 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Anabaena PCC 7122, Anabaena flos-aquae B1444, Anabaena cylindirca, Anabaena inaequalis B381, Calothrix parietina OB 1952, Coccomyxa subellipsoidea, Fremyella diplosiphon B481, Fischerella muscicola LB1829, Thermosynechococcus elongatus

PsaF | PSI-F subunit of photosystem I (cyanobacterial)

Product Information

Immunogen cyanobacterial recombinant PsaF, overexpressed in E.coli UniProt: P0A401

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 5000 - 1: 10 000 (WB)

Expected | apparent MW 17,6 | 15 kDa

Reactivity

Confirmed reactivity Anabaena PCC 7122, Anabaena flos-aquae B1444, Anabaena cylindirca, Anabaena inaequalis B381, Calothrix parietina OB 1952, Coccomyxa subellipsoidea, Fremyella diplosiphon B481, Fischerella muscicola LB1829, Thermosynechococcus elongatus

Not reactive in higher plants

Application examples

Additional information

Whole cell cyanobacterial (concentrated liquid culture) were mixed with SSB, heated, spun down and remaining supernatant was analysed by SDS-PAGE

Background

PsaF (PSI-F) is a conserved subunit of type I photosynthetic reaction centers (Photosystem I, PSI). PSI is an integral membrane multi-protein complex that catalyzes the electron transfer from plastocyanin (or cytochrome c6) to ferredoxin (or flavodoxin).

Product citations

Selected references To be added when available, antibody released in November 2020.

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additional information (application):	Whole cell cyanobacterial (concentrated liquid culture) were mixed with SSB, heated, spun down and remaining supernatant was analysed by SDS-PAGE
background:	PsaF (PSI-F) is a conserved subunit of type I photosynthetic reaction centers (Photosystem I, PSI). PSI is an integral membrane multi-protein complex that catalyzes the electron transfer from plastocyanin (or cytochrome c6) to ferredoxin (or flavodoxin).
Confirmed reactivity:	Anabaena PCC 7122, Anabaena flos-aquae B1444, Anabaena cylindirca, Anabaena inaequalis B381, Calothrix parietina OB 1952, Coccomyxa subellipsoidea, Fremyella diplosiphon B481, Fischerella muscicola LB1829, Thermosynechococcus elongatus
not reactive in:	higher plants
calculated \| apparent molecular mass [kDa]:	17,6 \| 15 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	cyanobacterial recombinant PsaF, overexpressed in E.coli UniProt: P0A401
Purity:	Serum
Quantity:	50 µl
recommended dilution:	1 : 5000 - 1: 10 000 (WB)
Reconstitution:	For reconstitution add 50 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
All references:	To be added when available, antibody released in November 2020.