PsaE | PSI-E subunit of photosystem I (monocot)

Product no: AS04 047

AS04 047  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Hordeum vulgare, Oryza sativa

390
Add to cart
Delivery:  3-6 business days
  • Product Info
  • Immunogen:

    10 kDa PSI-E protein purified from barley thylakoids corresponding to PSI-E protein of Horderum vulgare, UniProt: P13194

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Total IgG. Protein G purified in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 100 l
    Reconstitution: For reconstitution add 100 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 2000-1 : 5000 (WB)
    Expected | apparent MW: 10,8 | 10,5 kDa
  • Reactivity
  • Confirmed reactivity: Hordeum vulgare, Oryza sativa
    Predicted reactivity: Catalpa bungei, Nicotiana sylvestris, Zea mays
    Species of your interest not listed? Contact us
    Not reactive in:

    Arabidopsis thaliana, cyanobacteria, Chlamydomonas reinhardtii, Synechococcus sp. PCC7942

  • Application Examples
  • Application example


    Western blot detection using anti-PsaE antibodies

    2 µg of total protein
    from Arabidopsis thaliana leaf (1), Horderum vulgare leaf (2)Chlamydomonas reinhardtii total cell (3), Synechococcus sp.PCC 7942 total cell (4), all extracted with PEB (AS08 300) were separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% blocking reagent in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:20 000 in 2% blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with chemiluminescence detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).

  • Background
  • Background:

    PsaE is a nucleus encoded subunit of the Photosystem I reaction center. It is located on the stroma side and interacts with PsaF. PsaE may be involved in Fd reduction. Alternative name: Photosystem I 10.8 kDa polypeptide

  • Product Citations
  • Selected references: Yoshida et al. (2016). Hisabori T1.Two distinct redox cascades cooperatively regulate chloroplast functions and sustain plant viability. Proc Natl Acad Sci U S A. 2016 Jul 5;113(27):E3967-76. doi: 10.1073/pnas.1604101113. Epub 2016 Jun 22.
    Ye et al. (2012). A Mutation of OSOTP 51 Leads to Impairment of Photosystem I Complex Assembly and Serious Photo-damage in Rice. J Integr Plant Biol. Feb 2012.
    Yadavalli et al. (2012). Differential degradation of photosystem I subunits under iron deficiency in rice. J Plant Physiol. March 22.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts
  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
    Your name will be displayed as the sender.
    Number of reviews: (1)
    Mathias Pribil | 2011-09-15
    0 | 0
    This antibody works well with Zea mays. PsaE is detectable in mesophyll cell preparations as well as bundle sheath cells There are minor unspecific bands detectable.

Accessories

AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

213 €
Add to cart
Info

AS09 607 |  Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)

218 €
Add to cart
Info
img missing
AS16 ECL-N |  low pico to mid femtogram detection

This product can be purchased in 3 different volumes:

AS16 ECL-N-10, 10 ml. Trial size limited to one per customer

AS16 ECL-N-100, 100 ml

AS16 ECL-N-1L, 1L

Choose the appropriate volume in the drop down menu to the right

From 13 €
Close