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PsaG | PSI-G subunit of photosystem I

AS20 4368  | Clonality: Polyclonal  |  Host: Chicken  | Reactivity: Arabidopsis thaliana

PsaG | PSI-G subunit of photosystem I in the group Antibodies for Plant/Algal  / Photosynthesis  / PSI (Photosystem I) at Agrisera AB (Antibodies for research) (AS20 4368)

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Product Information

Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana PsaG, UniProt: Q9S7N7, TAIR: AT1G55670
Host Chicken
Clonality Polyclonal
Purity Affinity purified serum, in PBS pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl, of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW 17 | 11 kDa (after transit peptide is removed)

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Medicago truncatula, Spinacia oleracea, Vitis vinifera
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Western blot using anti-PsaG antibodies

4 µg/well of total chlorophyll from frozen thylakoids extracted freshly from Arabidopsis thaliana as described in Bassi et al., 1985 were separated on 4-15 % SDS-PAGE and blotted 1h to PVDF semi-dry. Blot was blocked with 5% milk for 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 in TBS-T ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-chicken IgG horse radish peroxidase conjugated AS010 1489) diluted to 1:5 000 in  for 1h/RT with agitation. The blot was washed as above and developed for 1 min with Agrisera ECLBright. Exposure time was 2 seconds.

Courtesy of Dr. Guillem Borràs I Gas, University of Copenhagen, Danmark

Additional information

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Background

Background PsaG (PSI-G subunit of photosystem I) is a 11-kDa membrane protein that plays an important role in electron transport between plastocyanin and PSI and is involved in the stability of the PSI complex. PSI-G subunit is bound to PSI-B and is in contact with Lhca1. The protein inserts into thylakoids by a direct or "spontaneous" pathway that does not involve the activities of any known chloroplast protein-targeting machinery. PSI-G appears to be directly or indirectly involved in the interaction between Photosystem I and plastocyanin.

Product citations

Selected references To be added when available, antibody released in November 2020.

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