PC | Plastocyanin

AS06 141 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, B. juncea, H. annuus, N. tabacum, O. sativa, P. sativum, S. oleracea, S. tuberosum, Z. mays | cellular [compartment marker] of chloroplast thylakoid lumen

Product Information

Immunogen

Purified native plastocyanin from Spinacia oleracea UniProt: P00289

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Immunogold (IG), Western blot (WB)

Recommended dilution 1 : 100 (IG), 1 : 2000 (WB)

Expected | apparent MW

10 kDa

Reactivity

Confirmed reactivity Arabidops thaliana, Brassica juncea, Heliantus annuus, Nicotiana tabacum, Oryza sativa, Pisum sativum, Spinacia oleracea, Solanum tuberosum, Zea mays

Predicted reactivity

Catalpa bungei, Dicots, Hordeum vulgare, Physcomitrella patens, Ricinus communis, Solanum lycopersicum

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example

western blot image using anti-plastocyanin antibody
10 µg of total protein from Arabidopsis thaliana (1), Brassica juncea (2), Zea mays (3), Oryza sativa (4), Solamum lycopersicum (5), Nicotiana tabacum (6), Heliantus annuus (7) were separated on SDS-PAGE and blotted to nitrocellulose. Filters were probed with anti-PC antibody (AS06 141, 1:2000). Signal was developed using alkaline phosphatase conjugated secondary antibody. Each sample was run in duplicate. Signal was developed using alkaline conjugated secondary antibody.

This antibody will also work well with HRP-conjugated secondary antibodies, as AS09 602.

Additional information

Cellular [compartment marker] of chloroplast thylakoid lumen

This product can be sold containing ProClin if requested.

Plastocyanin runs abberant due to negative charge at 12-19 kDa on SDS-PAGE depending upon the system used. in 15 % gel the protein will run closer to its true MW than in 12 % gel. In some cases PC can be very acidic and run at twice of its MW.

PC1 runs closer to 14 kDa while PC2 runs closer to 19 kDa. For good resolution adding fresh DTT to the sample buffer is recommended.
PC2 is generally more abundant and it increases with Cu feeding. PC1 is expressed first after etiolated seedlings are placed in the light.

Related products

AS06 202 | Anti-cytc6 marker antibody of thylakoid lumen for Chlamydomonas reinhardtii, rabbit antibodies

Plant protein extraction buffer

Secondary antibodies

Background

Plastocyanin (PC) is a small Cu protein and a mobile electron carrier in the lumen of the thylkoids. PC interacts with the B/F complex and Photosystem I. Alternative name: DNA-damage-repair/toleration protein DRT112.

Product citations

Selected references Cha et al. (2019). Arabidopsis GIGANTEA negatively regulates chloroplast biogenesis and resistance to herbicide butafenacil. Plant Cell Rep. 2019 Jul;38(7):793-801. doi: 10.1007/s00299-019-02409-x.
Mermod et al. (2019). SQUAMOSA promoter-binding protein-like 7 mediates copper deficiency response in the presence of high nitrogen in Arabidopsis thaliana. Plant Cell Rep. 2019 May 15. doi: 10.1007/s00299-019-02422-0.
Balyan et al. (2017). Identification of miRNA-mediated drought responsive multi-tiered regulatory network in drought tolerant rice, Nagina 22. Sci Rep. 2017 Nov 13;7(1):15446. doi: 10.1038/s41598-017-15450-1.
Perea-García et al. (2017). Arabidopsis copper transport protein COPT2 participates in the cross talk between iron deficiency responses and low-phosphate signaling. Plant Physiol. 2013 May;162(1):180-94. doi: 10.1104/pp.112.212407.
Yoshida et al. (2016). Hisabori T1.Two distinct redox cascades cooperatively regulate chloroplast functions and sustain plant viability. Proc Natl Acad Sci U S A. 2016 Jul 5;113(27):E3967-76. doi: 10.1073/pnas.1604101113. Epub 2016 Jun 22.
Kropat et al. (2015). Copper economy in Chlamydomonas: Prioritized allocation and reallocation of copper to respiration vs. photosynthesis. Proc Natl Acad Sci U S A. 2015 Feb 2. pii: 201422492.
Sook Seok et al. (2013). AtFKBP16-1, a chloroplast lumenal immunophilin, mediates response to photosynthetic stress by regulating PsaL stability. Physiologia Plantarum, DOI: 10.1111/ppl.12116.
Perera-Garcia et al. (2013). Arabidopsis copper transport protein COPT2 participates in the crosstalk between iron deficiency responses and low phosphate signaling.