Name:
Phone:
E-mail:
Message


 


Cyt b6 / PetB | Thylakoid membrane cytochrome b6 protein, N terminal

AS18 4169 | Clonality: Polyclonal  |  Host: Rabbit | Reactivity: A. thaliana, Echinochloa crus-galli , Panicum miliaceum, P. sativum, Z. mays

Cyt b6 / PetB | Thylakoid membrane cytochrome b6 protein, N terminal in the group Antibodies for Plant/Algal  / Photosynthesis  / Electron transfer at Agrisera AB (Antibodies for research) (AS18 4169)

DATA SHEET IN PDF

Qty: 
286
Buy 2 items of this product for 214.00 €/items
Buy 3 items of this product for 195.00 €/items
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen KLH-conjugated peptide chosen from Arabidopsis thaliana PetB protein sequence, UniProt: P56773, TAIR: ATCG00720
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl, of sterile water.
Storage Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks.
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 - 1: 5000 (WB)
Expected | apparent MW 24 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Echinochloa crus-galli, Panicum miliaceum, Pisum sativum, Zea mays
Predicted reactivity Acacia prainii, Angelphytum bahiense, Aristolochia promissa, Daucus glochidiatus, Dimerostemma brasilianum, Elaphandra paucipunctata, Fraxinus chiisanensis, Gossypium populifolium, Hydrangea heteromalla, Nicotiana sylvestris, Oryza sativa, Salix tetrasperma, Solanum lycopersicum, Thottea siliquosa, Triticum turgidum, Ulva flexuosa, Wedelia biflora, Vanilla aphylla
Species of your interest is not listed? Species of your interest not listed? Contact us.

Species of your interest not listed? Contact us
Not reactive in Chlamydomonas reihardtii

Application examples

Application examples Application example

Western blot using anti-PetB antibodies



0.75 µg of chlorophyll from Pisum sativum thylakoids and from mesophyll (M) and bundle sheath (BS) thylakoids of Zea mays, Panicum miliaceum, Echinochloa crus-galli  extracted with 0.4 M sorbitol, 50 mM Hepes NaOH, pH 7.8, 10 mM NaCl, 5 mM MgCl2 and 2 mM EDTA were loaded to lanes. Samples were denatured with Laemmli buffer at 75 °C for 5 min and were separated on 12% SDS-PAGE and blotted 30 min to PVDF using wet transfer. Blot was blocked with 5% milk for 2h at room temperature (RT) with agitation. Blot was incubated in the primary antibody  a dilution of 1: 1000 overnight at 4°C with agitation in 1% milk in TBS-T. The antibody solution was decanted and the blot was washed 4 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG HRP conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in 1 % milk in TBS-T for 1h at RT with agitation. The blot was washed 5 times for 5 min in TBS-T and 2 times for 5 min in TBS, and developed for 1 min with 1.25 mM luminol, 0.198 mM coumaric acid and 0.009% H2O2 in 0.1 M Tris- HCl, pH 8.5. Exposure time in ChemiDoc System was 54 seconds.

Courtesy Dr. Wioleta Wasilewska-Dębowska, Warsaw University, Poland

Additional information

Related products

Related products AS06 119 | anti-Cyt f | cytochrome f protein (PetA) of thylakoid Cyt b6/f-complex, rabbit antibodies
AS08 306 | Anti-Cyt f | cytochrome f protein (PetA) of thylakoid Cyt b6/f-complex, rabbit antibodie
AS08 330 | Anti-PetC | Rieske iron-sulfur protein of Cyt b6/f complex, rabbit antibodies

collection of antibodies to proteins of photosynthetic electron transfer

Background

Background Multi-subunit complex of cytb6/f is a crucial component for the photosynthetic electron transport chain of higher plants, green algae and cyanobacteria. This complex is catalyzing oxidation of quinols and the reduction the reduction of plastocyanin. This reaction allows to establish the proton force required for the ATP synthesis. Four major subunits build the complex: the petA gene product corresponding to a c-type cytochrome (cytf), the petB gene product corresponding to a b-type/c?-type cytochrome with three haems (cyt b6), the petD gene product (subunit IV, or suIV), and the petC gene product, corresponding to the Rieske/Iron/sulfur protein.

Product citations

Selected references Wang et al. (2020). Post-translational coordination of chlorophyll biosynthesis and breakdown by BCMs maintains chlorophyll homeostasis during leaf development. Nat Commun. 2020; 11: 1254.

Related products: Cyt b6 / PetB | Thylakoid membrane cytochrome b6 protein, N terminal

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
From 15 €
AS09 607 Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)
195 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
190 €
AS08 330 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, B. rapa subsp. chinensi...
286 €
Buy 2 items of this product for 214.00 €/items
Buy 3 items of this product for 195.00 €/items