Cyt b6 / PetB | Thylakoid membrane cytochrome b6 protein, N terminal

AS18 4169 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, Echinochloa crus-galli, N. oceanica, Panicum miliaceum, P. sativum, Z. mays

Cyt b6 / PetB | Thylakoid membrane cytochrome b6 protein, N terminal

Product Information

Immunogen KLH-conjugated peptide chosen from Arabidopsis thaliana PetB protein sequence, UniProt: P56773, TAIR: ATCG00720

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µg

Reconstitution For reconstitution add 50 µl, of sterile water

Storage Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Blue native PAGE (BN-PAGE), Western blot (WB)

Recommended dilution 1 : 1000 - 1: 5000 (WB)

Expected | apparent MW 24 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Echinochloa crus-galli, Nannochloropsis oceanica strain IMET1, Panicum miliaceum, Pisum sativum, Zea mays

Predicted reactivity Acacia prainii, Angelphytum bahiense, Aristolochia promissa, Chlamydomonas reinhardii, Daucus glochidiatus, Dimerostemma brasilianum, Elaphandra paucipunctata, Fraxinus chiisanensis, Gossypium populifolium, Hydrangea heteromalla, Nicotiana sylvestris, Oryza sativa, Salix tetrasperma, Solanum lycopersicum, Thottea siliquosa, Triticum turgidum, Ulva flexuosa, Wedelia biflora, Vanilla aphylla
Species of your interest is not listed? Species of your interest not listed? Contact us.

Species of your interest not listed? Contact us

Not reactive in Chlamydomonas reihardtii

Application examples

Application examples Application example

Western blot using anti-PetB antibodies

0.75 µg of chlorophyll from Pisum sativum thylakoids and from mesophyll (M) and bundle sheath (BS) thylakoids of Zea mays, Panicum miliaceum, Echinochloa crus-galli extracted with 0.4 M sorbitol, 50 mM Hepes NaOH, pH 7.8, 10 mM NaCl, 5 mM MgCl₂ and 2 mM EDTA were loaded to lanes. Samples were denatured with Laemmli buffer at 75 °C for 5 min and were separated on 12% SDS-PAGE and blotted 30 min to PVDF using wet transfer. Blot was blocked with 5% milk for 2h at room temperature (RT) with agitation. Blot was incubated in the primary antibody a dilution of 1: 1000 overnight at 4°C with agitation in 1% milk in TBS-T. The antibody solution was decanted and the blot was washed 4 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG HRP conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in 1 % milk in TBS-T for 1h at RT with agitation. The blot was washed 5 times for 5 min in TBS-T and 2 times for 5 min in TBS, and developed for 1 min with 1.25 mM luminol, 0.198 mM coumaric acid and 0.009% H₂O₂ in 0.1 M Tris- HCl, pH 8.5. Exposure time in ChemiDoc System was 54 seconds.

Courtesy Dr. Wioleta Wasilewska-Dębowska, Warsaw University, Poland

Additional information

Background

Background Multi-subunit complex of cytb6/f is a crucial component for the photosynthetic electron transport chain of higher plants, green algae and cyanobacteria. This complex is catalyzing oxidation of quinols and the reduction the reduction of plastocyanin. This reaction allows to establish the proton force required for the ATP synthesis. Four major subunits build the complex: the petA gene product corresponding to a c-type cytochrome (cytf), the petB gene product corresponding to a b-type/c?-type cytochrome with three haems (cyt b6), the petD gene product (subunit IV, or suIV), and the petC gene product, corresponding to the Rieske/Iron/sulfur protein.

Product citations

Selected references Lu et al. (2021). Role of an ancient light-harvesting protein of PSI in light absorption and photoprotection. Nat Commun. 2021 Jan 29;12(1):679. doi: 10.1038/s41467-021-20967-1. PMID: 33514722; PMCID: PMC7846763.
Chen et al. (2021)Degradation of the photosystem II core complex is independent of chlorophyll degradation mediated by Stay-Green Mg2+ dechelatase in Arabidopsis,Plant Science,Volume 307,2021,110902,ISSN 0168-9452,https://doi.org/10.1016/j.plantsci.2021.110902.
Wada et al. (2021) Identification of a Novel Mutation Exacerbated the PSI Photoinhibition in pgr5/pgrl1 Mutants; Caution for Overestimation of the Phenotypes in Arabidopsis pgr5-1 Mutant. Cells. 2021 Oct 26;10(11):2884. doi: 10.3390/cells10112884. PMID: 34831107; PMCID: PMC8616342.
Wang et al. (2020). Post-translational coordination of chlorophyll biosynthesis and breakdown by BCMs maintains chlorophyll homeostasis during leaf development. Nat Commun. 2020; 11: 1254.