PetC | Rieske iron-sulfur protein of Cyt b6/f complex

AS08 330 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, B. rapa subsp. chinensis, C. reinhardtii, E. crus-galli, Euglena sp., H. pluvialis, N. tabacum, P. miliaceum, P. sativum, S. oleracea, Synechococcus PCC 7942, Synechocystis sp. PCC 6803, Thalassiosira guillardii, Z. mays

Product Information

Immunogen

KLH-conjugated synthetic peptide which shows strong conservation across higher plants including Arabidopsis thaliana UniProt:Q9ZR03, TAIR:At4g03280, Chlamydomonas reinhardtii P49728 and Synechococcus sp. Q5N5B0

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Blue Native PAGE (BN-PAGE), Western blot (WB)

Recommended dilution 1 : 5000-1 : 10 000 (BN-PAGE), (WB)

Expected | apparent MW

23 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Brassica rapa subsp. chinensis, Chlamydomonas reinhardtii, Echinola crus-galli, Euglena sp., Haematococcus pluvialis, Nicotiana tabacum, Panicum miliaceum, Pisum sativum, Spinacia oleracea, Synechococcus PCC 7942, Synechocystis sp. PCC 6803, Thalassiosira guillardii, Zea mays

Predicted reactivity

Acetabularia acetabulum, Brachypodium distachyon, cyanobacteria, Calothrix sp. PCC 7507, Catalpa bungei, Cicer arietinum, Crocosphaera watsonii, Cynodon dactylon, Gossypium raimondii , Hordeum vulgare, Lyngbya aestuarii,Microcystis aeruginosa, Nannochloropsis gaditana, Nicotiana tabacum, Pisum sativum, Ricinus communis , Saccharum hybrid cultivar ROC22, Selaginella moellendorffii, Solanum tuberosum, Sorghum bicolor, Oryza sativa, Physcomitrella patens, Phormidesmis priestleyi, Populus trichocarpa, Sonneratia alba, Triticum aestivum, Zostera marina, Vitis vinifera

Species of your interest not listed? Contact us

Not reactive in Candidia albicans

Application examples

Application example

5 µg of total protein from (1) Arabidopsis thaliana leaf extracted with Protein Extration Buffer, PEB (AS08 300), (2) Euglena sp. extracted with PEB, (3) Synechococcus elongatus whole cell extracted with PEB, were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% blocking reagent 0.1% (v/v) Tween-20 (TBS-T) for 1h/RT with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 for 1h/RT with agitation. The blots were washed as above and developed for 5 min withchemiluminescence detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).

Additional information

This product can be sold containing Proclin if requested.

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AS08 330S | PetC | Rieske iron-sulfur protein of Cyt b6/f complex, protein standard for quantitation of PetC protein

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Background

Rieske Iron-Sulfur Protein (Q9ZR03) is located in chloroplast thylakoid membrane as a component of cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions. Alternative names: Rieske iron-sulfur protein, RISP, ISP, plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein, proton gradient regulation protein 1

Product citations

Selected references Pipitone et al. (2021). A multifaceted analysis reveals two distinct phases of chloroplast biogenesis during de-etiolation in Arabidopsis. Elife. 2021 Feb 25;10:e62709. doi: 10.7554/eLife.62709. PMID: 33629953; PMCID: PMC7906606.
Kana et al. (2020). Fast Diffusion of the Unassembled PetC1-GFP Protein in the Cyanobacterial Thylakoid Membrane. Life (Basel). 2020 Dec 29;11(1):E15. doi: 10.3390/life11010015. PMID: 33383642.
Zhang et al. (2020). Enhanced Relative Electron Transport Rate Contributes To Increased Photosynthetic Capacity In Autotetraploid Pak Choi. Plant Cell Physiol. 2020 Jan 6. pii: pcz238. doi: 10.1093/pcp/pcz238.
Pralon et al. (2019). Plastoquinone homoeostasis by Arabidopsis proton gradient regulation 6 is essential for photosynthetic efficiency. Commun Biol. 2019 Jun 20;2:220. doi: 10.1038/s42003-019-0477-4.
Koochak et al. (2019). The structural and functional domains of plant thylakoid membranes. Plant J. 2019 Feb;97(3):412-429. doi: 10.1111/tpj.14127.
Liang et al. (2018). Thylakoid-Bound Polysomes and a Dynamin-Related Protein, FZL, Mediate Critical Stages of the Linear Chloroplast Biogenesis Program in Greening Arabidopsis Cotyledons. Plant Cell. 2018 Jul;30(7):1476-1495. doi: 10.1105/tpc.17.00972. Epub 2018 Jun 7.
Koochak et al. (2018). The structural and functional domains of plant thylakoid membranes. Plant J. 2018 Oct 12. doi: 10.1111/tpj.14127.(Blue Native PAGE)
Du et al. (2018). Galactoglycerolipid Lipase PGD1 Is Involved in Thylakoid Membrane Remodeling in Response to Adverse Environmental Conditions in Chlamydomonas. Plant Cell. 2018 Feb;30(2):447-465. doi: 10.1105/tpc.17.00446.
Wood et al. (2018). Dynamic thylakoid stacking regulates the balance between linear and cyclic photosynthetic electron transfer. Nat Plants. 2018 Feb;4(2):116-127. doi: 10.1038/s41477-017-0092-7.
Schöttler et al. (2017). The plastid-encoded PsaI subunit stabilizes photosystem I during leaf senescence in tobacco. J Exp Bot. 2017 Feb 1;68(5):1137-1155. doi: 10.1093/jxb/erx009.
Xing et al. (2017). Deletion of CGLD1 Impairs PSII and Increases Singlet Oxygen Tolerance of Green Alga Chlamydomonas reinhardtii. Front. Plant Sci., 15 December 2017.
Zang et al. (2017). Characterization of the sulfur-formation (suf) genes in Synechocystis sp. PCC 6803 under photoautotrophic and heterotrophic growth conditions. Planta. 2017 Jul 14. doi: 10.1007/s00425-017-2738-0.
Nath et al. (2016). A Nitrogen-Fixing Subunit Essential for Accumulating 4Fe-4S-Containing Photosystem I Core Proteins. Plant Physiol. 2016 Dec;172(4):2459-2470. Epub 2016 Oct 26.
Zhang et al. (2016). A new paradigm for producing astaxanthin from the unicellular green alga Haematococcus pluvialis. Biotechnol Bioeng. 2016 Oct;113(10):2088-99. doi: 10.1002/bit.25976. Epub 2016 Mar 28.
Fristedt et al. (2015). The thylakoid membrane protein CGL160 supports CF1CF0 ATP synthase accumulation in Arabidopsis thaliana. PLoS One. 2015 Apr 2;10(4):e0121658. doi: 10.1371/journal.pone.0121658.

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