PetC | Rieske iron-sulfur protein of Cyt b6/f complex, protein standard
AS08 330S | recombinant protein standard for quantitation
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Standard curve: 3 loads are recommended (0.5, 2 and 4μl).
Positive control:a 2μl load per well is optimal for most chemiluminescent detection systems.
|Expected | apparent MW||
33 kDa (larger than native protein due to the addition of His-tag). In most gel systems, PetC protein migrates at 23 kDa
|Not reactive in|
Concentration: after adding 225 µl of milliQ water final concentration of the standard is 0.15 pmol/µl
Protein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.
This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.
|Selected references||Wu et al. (2014). Large centric diatoms allocate more cellular nitrogen to photosynthesis to counter slower RUBISCO turnover rates. Front. Mar. Sci., 09 December 2014 | doi: 10.3389/fmars.2014.00068.
Li et al. (2014). The nitrogen costs of photosynthesis in a diatom under current and future pCO2. New Phytol. 2014 Sep 25. doi: 10.1111/nph.13037.
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