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AGO set
Tag Antibody Set 

PsaF | PSI-F subunit of photosystem I

AS06 104  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana, H. vulgare, N. tabaccum, O. sativa, S. oleracea

PsaF | PSI-F subunit of photosystem I in the group Antibodies for Plant/Algal  / Photosynthesis  / PSI (Photosystem I) at Agrisera AB (Antibodies for research) (AS06 104)

PRODUCT INFORMATION IN PDF


Datasheet Product citations Protocols Customer reviews

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from the PsaF protein sequence of Arabidopsis thaliana (At1g31330). This peptide sequence is not completely conserved in mono- and dicots.

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

24 kDa | 17 kDa for Arabidopsis thaliana

Reactivity

Confirmed reactivity Arabidopsis thaliana, Briopsis corticulans, Hordeum vulgare (very weak), Nicotiana tabaccum, Oryza sativa, Spinacia oleracea
Predicted reactivity Catalpa bungei, Micromonas sp. , Populus trichocarpa, Physcomitrella patens, Ricinus communis
Not reactive in

Chlamydomonas reinhardtii, Synechococcus PCC 7942

Application examples

Application examples

Application example

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2 µg of total leaf protein of Arabidopsis thaliana (1) and Hordeum vulgare (2) and total cellular protein of Chlamydomonas reinhardtii (3) and Synechococcus PCC 7942 (4) isolated with PEB (AS08 300) were separated on 4-12% Nupage Bis-Tris gels in in MES running buffer (Invitrogen) at 200V for 35 minutes. Proteins were transferred for 80 minutes at 30V to a PVDF membrane pre-wetted in methanol and equilibrated in 1X transfer buffer. Blots were blocked immediately following transfer in 2% blocking reagent in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) and probedwith anti-PsaF (AS06 104, 1:1000) and secondary HRP-conjugated goat anti-rabbit antibody (1:50 000) for 1 hr in TBS-T containing 2% ECL Advance blocking reagent (GE Healthcare). Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signals was detected after 30 s using chemiluminescence detection reagent, according to the manufacturers instructions and a CCD imager (FluorSMax, Bio-Rad).

Additional information

Related products

Background

Background

PsaF (PSI-F) is a conserved subunit of type I photosynthetic reaction centers (Photosystem I, PSI). PSI is an integral membrane multi-protein complex that catalyzes the electron transfer from plastocyanin (or cytochrome c6) to ferredoxin (or flavodoxin). PsaF has been shown to be involved in the orientation of the soluble electron donor. In plants PSI-F is nuclear encoded and imported post-translationally into the chloroplast where it inserts into the thylakoid membrane.

Product citations

Selected references Patil et al. (2018). FZL is primarily localized to the inner chloroplast membrane however influences thylakoid maintenance. Plant Mol Biol. 2018 Jul;97(4-5):421-433. doi: 10.1007/s11103-018-0748-3.
Myouga et al. (2018). Stable accumulation of photosystem II requires ONE-HELIX PROTEIN1 (OHP1) of the light harvesting-like family. Plant Physiol. 2018 Feb 1. pii: pp.01782.2017. doi: 10.1104/pp.17.01782.
Kanazawa et al. (2017). Chloroplast ATP Synthase Modulation of the Thylakoid Proton Motive Force: Implications for Photosystem I and Photosystem II Photoprotection. Front Plant Sci. 2017 May 3;8:719. doi: 10.3389/fpls.2017.00719.
Qin et al. (2014). Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans. Photosynth Res. 2014 Sep 12.

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