PSA3 | Photosystem I Assembly 3
AS15 2872 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Zea mays

Data sheet | Product citations | Add review |
Product Information
Immunogen
Recombinant PSA3, amino acids 110 to 269 derived from Zea mays Zm00001d013295
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Quantity
50 ĩg
Reconstitution
For reconstitution add 50 ĩl, of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 2000 (WB)
Expected | apparent MW
26 kDa
Reactivity
Confirmed reactivity
Zea mays
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
Application example

5 µg of total protein from Zea mays leaf seedling tissue was extracted with protein extraction buffer (100 mM Tris pH7.5, 10% glycerol, 1 mM EDTA, 1mM EGTA, 2mM pMSF, 0.002 mg leupeptin, 0.002 mg pepstatin A, 2.5 mM DTT) and denatured in 1XPSB (33 mM Tris pH 6.8, 50 mM 2-Mercaptoethanol, 2% SDS, 10% glycerol, 0.1% bromophenol blue) at 70°C for 5 min, separated on 4-15% Tris-Glycine SDS-PAGE and blotted overnight to nitrocellulose using tank transfer. Blots were blocked with 4% milk in 1XTBST for 1h at room temperature (RT) with agitation. The blot was incubated in the primary antibody at a dilution of 1:2000 for 1h at RT with agitation in TBST. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBST at RT with agitation. The blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 for 1h at RT with agitation. The blot was washed as above and developed for 2 min with chemiluminescent detection reagent.
Courtesy Dr. Alice Barkan, University of Oregon, USA

5 µg of total protein from Zea mays leaf seedling tissue was extracted with protein extraction buffer (100 mM Tris pH7.5, 10% glycerol, 1 mM EDTA, 1mM EGTA, 2mM pMSF, 0.002 mg leupeptin, 0.002 mg pepstatin A, 2.5 mM DTT) and denatured in 1XPSB (33 mM Tris pH 6.8, 50 mM 2-Mercaptoethanol, 2% SDS, 10% glycerol, 0.1% bromophenol blue) at 70°C for 5 min, separated on 4-15% Tris-Glycine SDS-PAGE and blotted overnight to nitrocellulose using tank transfer. Blots were blocked with 4% milk in 1XTBST for 1h at room temperature (RT) with agitation. The blot was incubated in the primary antibody at a dilution of 1:2000 for 1h at RT with agitation in TBST. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBST at RT with agitation. The blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 for 1h at RT with agitation. The blot was washed as above and developed for 2 min with chemiluminescent detection reagent.
Courtesy Dr. Alice Barkan, University of Oregon, USA
Additional information
Background
Background
PSA3 (Photosystem I Assembly 3) is involved in promotion of photosystem I biogenesis in angiosperms. It is a nucleus-encoded protein.
Product citations
Selected references
Shen J, Williams-Carrier R, and Barkan A. (2017) PSA3, a protein on the stromal face of the thylakoid membrane, promotes photosystem I accumulation in cooperation with the assembly factor PYG7. Plant Physiol. 2017 Jul;174(3):1850-1862. doi: 10.1104/pp.17.00524.
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