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PSA3 | Photosystem I Assembly 3

AS15 2872  | Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Zea mays

PSA3 | Photosystem I Assembly 3 in the group Antibodies for Plant/Algal  / Photosynthesis  / PSI (Photosystem I) at Agrisera AB (Antibodies for research) (AS15 2872)

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Data sheet Product citations Add review

Product Information

Immunogen Recombinant PSA3, amino acids 110 to 269 derived from Zea mays  Zm00001d013295
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity

50 µg

Reconstitution For reconstitution add 50 µl, of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 2000 (WB)
Expected | apparent MW 26 kDa

Reactivity

Confirmed reactivity Zea mays
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example

western blot using anti-PSA3 antibodies

5 µg of total protein from Zea mays leaf seedling tissue was extracted with protein extraction buffer (100 mM Tris pH7.5, 10% glycerol, 1 mM EDTA, 1mM EGTA, 2mM pMSF, 0.002 mg leupeptin, 0.002 mg pepstatin A, 2.5 mM DTT) and denatured in 1XPSB (33 mM Tris pH 6.8, 50 mM 2-Mercaptoethanol, 2% SDS, 10% glycerol, 0.1% bromophenol blue) at 70°C for 5 min, separated on 4-15% Tris-Glycine SDS-PAGE and blotted overnight to nitrocellulose using tank transfer. Blots were blocked with 4% milk in 1XTBST for 1h at room temperature (RT) with agitation. The blot was incubated in the primary antibody at a dilution of 1:2000 for 1h at RT with agitation in TBST. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBST at RT with agitation. The blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 for 1h at RT with agitation. The blot was washed as above and developed for 2 min with chemiluminescent detection reagent.

Courtesy Dr. Alice Barkan, University of Oregon, USA

Additional information

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Background

Background PSA3 (Photosystem I Assembly 3) is involved in promotion of photosystem I biogenesis in angiosperms. It is a nucleus-encoded protein.

Product citations

Selected references Shen J, Williams-Carrier R, and Barkan A. (2017) PSA3, a protein on the stromal face of the thylakoid membrane, promotes photosystem I accumulation in cooperation with the assembly factor PYG7. Plant Physiol. 2017 Jul;174(3):1850-1862. doi: 10.1104/pp.17.00524.

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