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PsaL | PSI-L subunit of photosystem I (cyanobacterial)

AS19 4353 |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Calothrix sp., Thermocynechococcus elongatus

PsaL | PSI-L subunit of photosystem I (cyanobacterial) in the group Antibodies for Plant/Algal  / Photosynthesis  / PSI (Photosystem I) at Agrisera AB (Antibodies for research) (AS19 4353)

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Product Information

Immunogen Recombinant PsaL of Thermosynechococcus elongatus, overexpressed in E.coli, UniProt: Q8DGB4
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 3000 - 1: 5000 (WB)
Expected | apparent MW

16 kDa

Reactivity

Confirmed reactivity Calothrix sp., Synechococcus PCC6803, Thermosynechococcus elongatus
Predicted reactivity Species of your interest not listed? Contact us
Not reactive in higher plants

Application examples

Application examples Western blot using anti-PsaL antibodies

Samples:
1 - 10 μg of Synechocystis PCC 6803 isolated thylakoid membranes
2 - 20 μg of Synechocystis PCC 6803 isolated thylakoid membranes
3 - 10 μg of PsbE deletion mutant Synechocystis PCC 6803 isolated thylakoid membranes

10 or 20 µg/well of total protein from isolated thylakoid membranes with MES buffer (25mM MES/NaOH, pH 6.5, 10 mM MgCl2, 10 mM CaCl2, and 25% glycerol) and denatured with SDS-solubilizing buffer stock (5 % SDS in 25mM Tris-HCl, pH 7, 1M sucrose) with working concentrations 1% SDS and 2% DTT at 23°C for 1 hour were separated on 16 - 20 % AA, 7 M urea SDS-PAGE and blotted 1h to PVDF (pore size of 40 μm), using wet transfer. Blot was stripped with Re-Blot Plus (Antibody Stripping Solution, Merck) for 15 min/RT, blocked twice with 0.2 % Tween in TBS for 5 min/RT with agitation, washed once with TBS-T for 15 min/RT with agitation, and incubated in the PsaL primary antibody at a dilution of 1: 4 000 overnight at 4°C with agitation in TBS-T. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera secondary antibody (AS09 602) diluted to 1:25 000 in TBS-T for 1h/RT with agitation. The blot was washed as above, incubated for 5 min in AgriseraECL Bright (AS16 ECL-N) and developed in LAS-4000 camera (Fujifim, Japan). Exposure time was 1 minutes.

Courtesy Dr. Josef Komenda, ALGATECH, Czech Republic

Additional information

Related products

Background

Background

PsaL (PSI-L) is a conserved subunit of type I photosynthetic reaction centers (Photosystem I, PSI). PSI is an integral membrane multi-protein complex that catalyzes the electron transfer from plastocyanin (or cytochrome c6) to ferredoxin (or flavodoxin). Alternative names: PSI subunit V, PSI-L

Product citations

Selected references To be added when available, antibody released in November 2020.

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