BirA (mutated/TurboID) - DyLight® 488 conjugated (40 µg)

Product no: AS20 4440-DL488
AS20 4440-DL488 | Clonality: Polyclonal |  Host: Rabbit | Reactivity: E. coli BirA - mutated/TurboID overexpressed in various organisms
490 €
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Delivery:  3-6 business days
  • Data sheet
    • Product Info
    • Immunogen: Recombinant mutated BirA protein from E.coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177). Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag).
      Host: Rabbit
      Clonality: Polyclonal
      Purity:

      Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight® 488.
      Format: Liquid in PBS pH 7,4.
      Quantity: 40 µg
      Storage: Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years. Spin briefly the tube before use.
      Tested applications: Immunofluorescence (IF), Western blot (WB)
      Recommended dilution: To be determined by end user.
      Expected | apparent MW: Depends upon fusion partner
    • Reactivity
    • Confirmed reactivity: BirA (mutated/TurboID)
      Not reactive in: No confirmed exceptions from predicted reactivity are currently known,
    • Additional Information
    • Additional information: DyLight® 488 Amax = 493 nm, Emax = 519 nm. DyLight® is a registered trademark of Thermofisher Inc., and its subsidiaries.
    • Background
    • Background: Tagging a protein with TurboID allows studying protein interactions in different types of cells and organs and developmental stages, This is a suitable tool for proximity labelling experiments as described in Mair et al, (2019), Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID, Elife , 2019 Sep 19;8:e47864, doi: 10,7554/eLife,47864, This tag has MW of 35 kDa.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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