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Donkey anti-Sheep IgG (H&L), HRP conjugated

181 €

AS09 604 | clonality: polyclonal  |  host: donkey  | reactivity: sheep IgG (H&L)

PRODUCT INFORMATION IN PDF

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AS09 604

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product information
Background

Donkey anti-sheep IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all sheep immunoglobulins in immunological assays.

Immunogen

Purified sheep IgG, whole molecule

Host Donkey
Clonality Polyclonal
Clone
Purity Purified donkey IgG
Format Lyophilized
Quantity 1 mg
Reconstitution
Storage Store lyophilized material at 2-8°C. For storage at -20°C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC)
Related products

AS09 611 | Donkey anti-sheep IgG (H&L) biotin conjugated

Additional information

Antibody concentration is 1 mg/ml and it has been purified by antigen-specific chromatography

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free

0.1 % (v/v) of Kathon CG is used as preservative.

BSA and milk have to be replaced by other blocking reagents, like
doneky serum or commercial formulations which are free from bovine IgG.

application information
Recommended dilution

1: 200 -1: 5 000 (ELISA), 1:200-1:5 000 with regular ECL (WB), 1: 500 -1: 5000 (IHC)

Expected | apparent MW
Confirmed reactivity

sheep IgG heavy and light chains (H&L)

Predicted reactivity

sheep IgG heavy and light chains (H&L)

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

No reactivity is observed to non-immunoglobulin sheep serum proteins based in immunoelectrophoresis

Selected references

to be added when available



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