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Goat anti-Rat IgG (H&L), HRP conjugated, min. cross-reactivity to human, mouse lgG (highly absorbed against mouse IgG)

181 €

AS09 618 | clonality: polyclonal  |  host: goat  | reactivity: rat IgG (H&L)

PRODUCT INFORMATION IN PDF

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AS09 618

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product information
Background

Goat anti-rat IgG  is a secondary antibody conjugated to HRP which binds to all rabbit IgGs in immunological assays.

Immunogen

Purified Rat IgG, whole molecule

Host Goat
Clonality Polyclonal
Clone
Purity Purified goat IgG
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilutions daily.
Storage Store undiluted liquid at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA) , western blot (WB), immunohistochemistry (IHC)
Related products

AS09 619 | Goat anti-rat IgG (H&L) biotin conjugated

AS06 620 | Goat anti-rat IgG (H&L) ALP(alkaline phosphatase) conjugated

Additional information

HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free

0.1 % (v/v) of Kathon CG is used as preservative.

application information
Recommended dilution

1: 10 000 -1: 50 000 (ELISA), (WB), 1: 500 -1: 5000 (IHC)

Expected | apparent MW
Confirmed reactivity

rat IgG heavy and light chains (H&L) of all rat immunoglobulins

Predicted reactivity

rat IgG heavy and light chains (H&L) of all rat immunoglobulins

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

No reactivity is observed to non-immunoglobulin rat serum. Antibody has been absorbed against mouse IgG and no reactivity is observed to  human or mouse IgG based on immunoelectrophoresis.

Selected references

to be added when available



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