Plant/Algal cell biology / Conifers

product information

Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H,O,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD

overexpressed Chlamydomonas reinhardtii thioredoxine fusion protein A8IGH1, FeSOD excised from a gel piece

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS06 170 | anti-CSD2 | chloroplastic Cu/Zn superoxide dismutase

AS07 219 | anti-CCS | chloroplastic copper chaperone for SOD

AS09 524 | anti-MnSOD | manganese superoxide dismutase

Collection of antibodies to Arabidopsis proteins

Collection of antibodies to Chlamydomonas proteins

to be added when available

application information

1: 1500 to 1:5000 with alkaline phosphatase (WB)

25 | 22 kDa

Armeria maritima, Arabidopsis thaliana, Chlamydomonas reinhardtii, Zea mays

dicots including: Glycine max, Solanum lycopersicum, trees: Pinus pinaster, Populus balsamifera, moss: Physcomitrella patens, algae including: Dunaliella salina, Volvox carteri

cyanobacteria

The antibody will detect FeSOD enzyme only in plants grown on low Cu (0.1 μM).Reference: Salah et al (2005) Two P-type ATPases are required for copper delivery in Arabidopsis thaliana chloroplasts. Plant Cell, 17, 1233-1251

to be added when available

application example

5 µg of stromal protein from (1) Chlamydomonas reinhardtii (left), (2) Arabidopsis thaliana   were separated on  SDS-PAGE. Primary antibodies have been used in 1: 3000.