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Plant/Algal cell antibodies / Conifers


FeSOD | chloroplastic Fe-dependent superoxide dismutase

Art no: AS06 125
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product information

background  

Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H,O,. SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD

immunogen  

overexpressed Chlamydomonas reinhardtii thioredoxine fusion protein A8IGH1, FeSOD excised from a gel piece

antibody format  

rabbit

polyclonal

serum

lyophilized

quantity  

200 µl

for reconstitution add 200 µl of sterile water.

storage  

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications  

western blot (WB)

related products  

AS06 170 | anti-CSD2 | chloroplastic Cu/Zn superoxide dismutase

AS07 219 | anti-CCS | chloroplastic copper chaperone for SOD

AS09 524 | anti-MnSOD | manganese superoxide dismutase

Collection of antibodies to Arabidopsis proteins

Collection of antibodies to Chlamydomonas proteins

additional information  

to be added when available

application information

recommended dilution  

1: 1500 to 1:5000 with alkaline phosphatase (WB)

expected | apparent MW  

25 | 22 kDa

confirmed reactivity  

Armeria maritima, Arabidopsis thaliana, Chlamydomonas reinhardtii, Zea mays

predicted reactivity  

dicots including: Glycine max, Solanum lycopersicum, trees: Pinus pinaster, Populus balsamifera, moss: Physcomitrella patens, algae including: Dunaliella salina, Volvox carteri

not reactive in  

cyanobacteria

additional information  

The antibody will detect FeSOD enzyme only in plants grown on low Cu (0.1 μM).Reference: Salah et al (2005) Two P-type ATPases are required for copper delivery in Arabidopsis thaliana chloroplasts. Plant Cell, 17, 1233-1251

selected references  

to be added when available


application example

 

5 µg of stromal protein from (1) Chlamydomonas reinhardtii (left), (2) Arabidopsis thaliana   were separated on  SDS-PAGE. Primary antibodies have been used in 1: 3000.  

 

Western blot using anti-FeSOD antibodies
 

||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com

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