Plant/Algal cell antibodies / Environmental stress / Heat shock

product information

Hsp21 is a chloroplast-localized small heat shock  protein. The way small hsp proteins are protecting a living cell are not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation.

recombinant Hsp21 protein derived from the sequence of Arabidopsis thaliana hsp21  P31170

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS08 284 | anti-HSP17.6 | cytosolic class I heat shock protein 17.6 hen antibody

AS08 254 | anti-HSP17.6 | cytosolic class I heat shock protein 17.6 rabbit antibody

AS07 255 | anti-HSP17.7 | cytosolic class II heat shock protein 17.7

collection of antibodies to plant heat shock proteins

recommended load per well 20 µg of total protein

application information

1: 3000 with standard ECL (WB)

25 | 21 kDa

Arabidopsis thaliana

Arabidopsis thaliana

no confirmed exceptions from predicted reactivity known in the moment

Please, note that there might be no HSPs accumulation below temperature of 32-34°C.  HSPs are induced when the plant experience temperatures higher than the growing temperature with around 10°C. So, the HSPs induction temperatures for plants grown at 18C differ from these for plants grown at 24C.

Another very effective parameter is the humidity. When using low humidity the plant has a chance to cool down through transpiration.  In this case the HSPs induction requires higher temperatures.

to be added when available