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product information
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| background |
|
Ricin is a protein found in castor bean (Ricinus communis) acting as protein synthesis inhibitor. It is a toxin involved in plant defence. A chain of ricin can inactivate few thousands of ribosomes per minute. B chain facilitates the entry of A chain into the cell. |
| immunogen |
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ricin, whole molecule |
| antibody format |
|
mouse |
monoclonal |
IgG1 |
purified IgG in phosphate buffer saline pH 7.4, contains mannitol |
lyophilized |
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| quantity |
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| storage |
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store at -70°C; avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. For storage at 4°C sodium azide can be added. |
| tested applications |
|
(ELISA), immunohistochemistry (IHC) |
| related products |
|
AS09 648 | ricin (RTA subunit), clone MMA collection of antibodies to food proteins |
| additional information |
|
Antibodies have been purified by affinity chromatography on Protein G from tissue culture supernatant. Ricin detection limit is 5 ng/ml, signal to noise ratio is 15-50 up to 100 ng/ml As detection antibody in sandwich-type ELISA, antibody, AS09 649, which is recongnizing a complimentary epitope is recommended. This product is conjugated. Suggested protocol can be found here. |
application information
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| recommended dilution |
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titer need to be determined by end user for specific application |
| expected | apparent MW |
|
29.8 kDa |
| confirmed reactivity |
|
Ricinus communis
|
| predicted reactivity |
|
Ricinus communis |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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to be added when available |
| selected references |
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to be added when available |
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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