Plant/Algal cell antibodies / Oryza sativa

product information

Hsp101/ClpB is a member of HSP100 protein family. These proteins help protein aggregates formed during heat stress to fall apart to allow them to be refolded by other chaperones. HSP101 is a cytosolic heat shock protein required for acclimation to high temperature.

recombinant Hsp101 N-terminal derived from the sequence of  Arabidopsis thaliana Hsp101 protein P42730

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS07 287 | anti-HSP101 | ClpB heat shock protein, C-terminal rabbit antibody

AS08 283 | anti-HSP101 | ClpB heat shock protein, N-terminal hen antibody

AS08 290 | anti-HSP101 | nuclear/cytoplasmatic heat shock proteinrabbit antibody with reactivity to Zea mays

collection of antibodies to plant heat shock proteins

to be added when available

application information

1: 1000 with standard ECL (WB)

101 kDa

Arabidopsis thaliana, Agave tequilana

dicots including: Glycine max, Nicotiana tabacum, Vitis vinifera, monocots including: Oryza sativa

no confirmed exceptions from predicted reactivity known in the moment

to be added when available

Vainonen et al. (2011). RCD1-DREB2A interaction in leaf senescence and stress responses in Arabidopsis thaliana. Biochem. J. Dec 12 (ahead of print).

Zhang et al. (2010). The role of Arabidopsis AtFes1A in cytosolic Hsp70 stability and abiotic stress tolerance. Plant J. 1;62(4):539-48.

Luján et al. (2009). Small heat-shock proteins and leaf cooling capacity account for the unusual heat tolerance of the central spike leaves in Agave tequilana var. Weber. Plant Cell Environ. 32(12):1791-803.

application example

2 µg of total protein from (1) Arabidopsis thaliana WT stressed at 38ºC for 1.5 hour, (2) Arabidopsis thaliana HSP101 null mutant (hot 1-3)  were separated on  7.5% SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 1 000 for 1h at room temperature with agitation and secondary HRP-conjugated antibody (1: 10 000 from Abcam).