Plant/Algal cell antibodies / Photosynthesis / Proteases

product information

Deg1 belongs to S1 chymotrypsin protease family and contains 16 protein members in Arabidopsis thaliana. Deg1 is localized to chloroplast.

recombinant Deg1 of Arabidopsis thaliana O22609, overexpressed in E.coli

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS10 703 | Deg5 | protease Do-like 5, chloroplastic

AS10 1581 | Deg2 | chloroplastic DegP-type serine protease 2

collection of antibodies to chloroplastic proteases

to be added when available

application information

1:500 on 5 ug thylakoid fraction (WB)

46.6 | 36 kDa

Arabidopsis thaliana, Pisum sativum, Zea mays

dicots including: Ricinus communis

no confirmed exceptions from predicted reactivity known in the moment

unspecific reaction at ca. 30 kDa and ca. 85 kDa (checked by by mass spec, and confirmed not to be neither Deg1 nor any other protease. The band at the right MW does contain Deg1 by our MS analysis

Pokorska et al. (2009). Differential turnover of the photosystem II reaction centre D1 protein in mesophyll and bundle sheath chloroplasts of maize. BBA, in press. Kapri-Pardes et al. (2007).  The thylakoid lumen protease Deg1 is involved in the repair of photosystem II from photoinhibition in Arabidopsis. Plant Cell 19:1039-1047.

application example

(1) Pisum sativum thylakoid fraction, (2) Arabidopsis thaliana thylakoid fraction, (3) recombinant Deg1 expressed in E.coli. Primary antibody has been used at 1: 500 dilution with standard ECL.