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product information
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| background |
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Goat anti-biotin is a secondary antibody conjugated to ALP, which binds to biotin in immunological assays. Antibody is affinity purified using solid phase biotin. |
| antibody format |
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| goat |
polyclonal, |
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affinity purified IgG, |
liquid |
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| quantity |
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| storage |
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Store undiluted liquid at 2-8°C. For storage at -20°C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard. Shelf life is 1 year from date of receipt. Prepare working dilution prior to use and then discard. |
| related products |
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other secondary antibodies against biotin
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| recommended dilution |
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This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:500 - 1:2000 for Immunohistochemistry 1:50 000 - 1:5 000 for ELISA/Western blot |
| additional information |
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Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.50 mg/ml (E 1% at 280 nm = 13.0). Antibody is supplied in 30 mM triethanolamine, pH 7.2.5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) B, Protease/IgG free. Contains 0.05% (w/v) sodium azide as preservative of bacterial growth.
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