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Suggested Control Antibodies for diatoms

AtpB | beta subunit of ATP synthase (10 µl) AS05 085-10 | clonality: polyclonal | host: rabbit 
This antibody works well and detects both the chloroplastic and the mitochondrial forms of the enzyme. It usually gives a tight band and shows good reactivity. With ECL Advance detection we usually load 1 ug of total protein from diatom extracts, using this antibody.
RbcL | Rubisco large subunit, form I and form II (10 µl)  AS03 037-10 | clonality: polyclonal | host: rabbit 
This antibody is very 'hot' and the RUBISCO target is abundant, so only a small load of total protein is required to get a total band. With ECL Advance we usually load 0.5 or even 0.25 ug of total protein from diatom extracts, using this antibody. So depending upon the strength of the antibody to which you are comparing, this anti-RUBISCO may be too 'hot' to be a good comparison.
PsbA | D1 protein of PSII, C-terminal (10 µl) AS05 084-10 | clonality: polyclonal | host: rabbit
This antibody is used if the comparison protein is a hydrophobic membrane protein. This antibody works well but the PsbA protein is subject to fragmentation upon storage and freeze-thaw cycles. This might be a good index of the 'quality' of the total protein extracts. For this antibody we normally load 1 ug total protein, when detecting using ECL Advance.
Note: TMA-6 from Lumigen gives similar results to ECL Advance.

Protocol for protein extraction from diatoms. 

Below you will find antibodies with confirmed and predicted reactivity to diatoms.


Agrisera Video Tutorial : western blot recommendations and trouble shooting News letter Protocols & technical information Protein extraction from diatoms
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100 €

AS08 300  |  extraction buffer for quantitative isolation of total soluble/membrane protein from plant tissue and algal/bacterial cells, optimized for subsequent western blot detection in denatured conditions

1324 €

SA000002  |  clonality: polyclonal  |  host: rabbit  |  reactivity: higher plants and algae

1310 €

AS05 070 | clonality: polyclonal | host: rabbit | reactivity: dicots, monocots, algae

873 €

AS04 051 | clonality: polyclonal | host: rabbit | reactivity: higher plants, algae, cyanobacteria

2647 €

SA000003 |  clonality: polyclonal  |  host: rabbit  |  reactivity: higher plants, algae

564 €

AS09 409 | clonality:polyclonal | host: rabbit | r eactivity: dicots, monocots, algae, cyanobacteria

174 €
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AS04 054S  |  protein standard for quantitation and positive control

272 €
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AS04 054  |  clonality: polyclonal  |  host: rabbit  |  reactivity: A. thaliana, B. nana, B. napus, B. vulgaris, E. vaginatum, H. vulgare, L. luteus, N.tabacum, O. sativa, P.abies, P. sativum, S.tuberosum and T.aestivum, conifers and P. patens cellular[compartment marker] of mitochondrial inner membrane

272 €
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AS15 2992  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Chlamydomonas reinhardtii

355 €

AS03 030  |  Clonality: Polyclonal  |  Host: Chicken  |  Reactivity: [global antibody] for plant and bacterial F-type ATP synthases

272 €
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AS05 085  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: [global antibody] for plant, green alga, animal and bacterial F-type ATP synthases

148 €

AS05 085-10  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: [global antibody] for plant, green alga and bacterial F-type ATP synthases

272 €
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AS16 3976  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana, Brassica oleracea var. botrytis cv. ’Diadom

272 €
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AS15 2991 | clonality: polyclonal | host: rabbit | reactivity: Chlamydomonas reinhardtii

348 €

AS10 935 | clonality: polyclonal | host: rabbit | reactivity: diatoms

 

272 €
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AS17 4116 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Heterosigma akashiwo

272 €
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AS11 1789   |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A.  acetabulum, A. thaliana, B. hypnoides (ulvophytes), G. theta, H. vulgare, Synechocystis sp., T. pseudonana, Z. mays

245 €

AS11 1789S  |  Protein standard for quantitation

Buy 2 positive controls/standards for the price of 1.
To get this discount please send your order to orders@agrisera.com
Follow this link to a list of discounted standards.


272 €
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AS13 2671 clonality: polyclonal host: chicken  reactivity: [global antibody] for di- and monocots, conifers, ferns, mosses, green algae cellular [compartment marker] for plasma membrane

174 €
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AS08 371S  |  protein standard for quantitation and positive control

332 €

AS08 282  |  clonality:polyclonal  |  host:rabbit  |  reactivity:diatoms

272 €
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AS17 4115 | clonality:polyclonal | host:rabbit | reactivity: Cyclotella sp.Thalassiosira pseudonana

381 €

AS01 021A  |  Clonality: Polyclonal  |  Host: Hen  |  reactivity: [global antibody]  for bacteria

407 €

AS08 310-100 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, H. vulgare, Ch. reinhardtii, red alga Gracilaria gracilis, diatom Thalassiosira sp. , P. tricornutum Bohlin, P. yunnanensis Dode, S. tuberosum

272 €
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AS08 310  |  Clonality: Polyclonal  |  Host: Rabbit   |  Reactivity: A. thaliana, H. vulgare, C. reinhardtii, red alga Gracilaria gracilis, diatom Thalassiosira sp., P. tricornutum Bohlin, P. yunnanensis Dode, S. lycopersicum, S. tuberosum

417 €

AS09 458-ALP | clonality:polyclonal | host:rabbit | reactivity: A. comosus, A. thaliana, S. PCC 6803, Ch. velia, H. vulgare, J. curcas, Lupinus sp. , M. crystallinum, N. tabacum, O. sativa, Saccharum spp. hybrid clone C91-301, S. laricifolia, Z. mays, Synechocystis sp. , P. tricornutum (strain CCAP 1055/1), T. weissfloggi

417 €

AS09 458-HRP | clonality:polyclonal | host:rabbit | reactivity: A. comosus, A. thaliana, S. PCC 6803, Ch. velia, H. vulgare, J. curcas, Lupinus sp. , M. crystallinum, N. tabacum, O. sativa, Saccharum spp. hybrid clone C91-301, S. laricifolia, Z. mays, Synechocystis sp. , P. tricornutum (strain CCAP 1055/1), T. weissfloggi

174 €
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AS09 458S | protein standard for quantitation and positive control

When you buy this product you get a 50% discount on other protein standards.
Follow this link to a list of discounted standards.
To get this discount please send your order to orders@agrisera.com

272 €
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AS09 458 | clonality:polyclonal | host:rabbit | reactivity: A. comosus, A. thaliana, S. PCC 6803, Ch. velia, F. bidentis, H. vulgare, J. curcas, Lupinus sp. , M. crystallinum, N. tabacum, O. sativa, P. strobus, Saccharum spp. hybrid clone C91-301, S. laricifolia, Z. mays, Synechocystis sp. , P. tricornutum (strain CCAP 1055/1), T. weissfloggi

AS15 2993 | clonality:polyclonal | host:rabbit | reactivity: Chlamydomonas reinhardtii

Antibody under development. Interested in testing? Please contact us.

272 €
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AS08 330 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, C. reinhardtii, E. crus-galli, Euglena sp., H. pluvialis, N. tabacum, P. miliaceum, P. sativumSynechococcus PCC 7942, Thalassiosira guillardii, Z. mays

245 €

AS08 330S  |  recombinant protein standard for quantitation

272 €
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AS07 257 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, H. vulgare, Z. mays, cyanobacteria, diatoms  | compartment marker of chloroplast stroma

417 €

AS07 257-ALP | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, H. vulgare, Z. mays, cyanobacteria, diatoms  | compartment marker of chloroplast stroma

417 €

AS07 257-HRP | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, Z. mays, cyanobacteria, diatoms  | compartment marker of chloroplast stroma

245 €

AS04 042S  |  recombinant protein standard for quantitation


272 €
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AS10 939  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: [global antibody] for higher plants, algae, cyanobacteria, diatoms

360 €

AS04 042P | clonality: polyclonal | host: rabbit | reactivity: [global antibody] for higher plants, algae, cyanobacteria

417 €

AS05 084A-ALP | clonality: polyclonal | host: rabbit | reactivity: [global antibody] for higher plants, algae, liverwort, cyanobacteria, diatoms | cellular [compartment marker] of thylakoid membrane

397 €

AS05 084A-HRP | clonality: polyclonal | host: rabbit | reactivity: [global antibody] for higher plants, algae, liverwort, cyanobacteria, diatoms | cellular [compartment marker] of thylakoid membrane

272 €
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AS05 084A  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: [global antibody] for higher plants, algae, liverwort, cyanobacteria, diatoms | Cellular [compartment marker] of thylakoid membrane

360 €

AS01 016  |  clonality: polyclonal  |  host: hen  |  reactivity: [global antibody] for higher plants, algae, cyanobacteria and dinoflagellates

272 €
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AS05 084 | clonality: polyclonal | host: rabbit | reactivity: [global antibody] for higher plants, algae, liverwort, cyanobacteria, diatoms | cellular [compartment marker] of thylakoid membrane

355 €

AS11 1786  | clonality: polyclonal  |  host: rabbit  |  reactivity: higher plants, alage, cyanobacteria

272 €
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AS13 2669 | clonality: polyclonal | host: rabbit | reactivity: [global antibody] for higher plants

360 €

AS04 038 | clonality: polyclonal | host: rabbit | reactivity: [global antibody] for higher plants, Physcomitrella patens, algae, cyanobacteria, diatoms

407 €

AS06 146-100  |  clonality: polyclonal  |  host: rabbit  |  reactivity: [global antibody] for A.thaliana, H. vulgare, L. corniculatus, N. tabacum, O. sativa, P. sativum, P. vulgaris, T. pratense, U. prolifera, C. reinhardtii, Synechococcus sp. PCC 7942, Anabaena 7120, diatoms

245 €

AS09 146S  |  Recombinant protein standard for quantitation and positive control

Buy 2 positive controls/standards for the price of 1.
To get this discount please send your order to orders@agrisera.com
Follow this link to a list of discounted standards.


272 €
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AS06 146  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: [global antibody] for A. thaliana, Anabaena 7120, D. brightwellii, H. vulgare, C.reinhardtii, L. corniculatus, N. tabacum, O. sativa, P. sativum, P. vulgaris, P. tricornutum, T. pratense, S. alba, Synechococcus sp. PCC 7942, Synechocystis sp. PCC6803, T. guillardii, T. pseudonana, U. prolifera

114 €

AS06 146PRE | host: rabbit

174 €
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AS15 2955S  |  protein standard for quantitation and positive control


272 €
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AS15 2955 |  clonality: polyclonal  |  host: rabbit  |  reactivity:  photosynthetic bacteria, archea, dinoflagellates

417 €

AS03 037-ALP clonality: polyclonal  |  host: rabbit  |  reactivity: [global antibody] for higher plants, lichens,  algae, cyanobacteria, dinoflagellates, diatoms  |  cellular [compartment marker] of plastid stroma in higher plants and cytoplasm in cyanobacteria

417 €

AS03 037B clonality: polyclonal  |  host: rabbit  |  reactivity: [global antibody] for higher plants, lichens,  algae, cyanobacteria, dinoflagellates, diatoms  |  cellular [compartment marker] of plastid stroma in higher plants and cytoplasm in cyanobacteria

417 €

AS03 037-HRP clonality: polyclonal  |  host: rabbit  |  reactivity: [global antibody] for higher plants, lichens,  algae, cyanobacteria, dinoflagellates, diatoms  |  cellular [compartment marker] of plastid stroma in higher plants and cytoplasm in cyanobacteria

272 €
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AS03 037A clonality: polyclonal  |  host: rabbit  |  reactivity: [global antibody] for higher plants, lichens,  algae, cyanobacteria, dinoflagellates, diatoms  |  cellular [compartment marker] of plastid stroma in higher plants and cytoplasm in cyanobacteria

265 €
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AS03 037 |  clonality: polyclonal  |  host: rabbit  |  reactivity: [global antibody] for higher plants, lichens, algae, cyanobacteria, dinoflagellates, diatoms  |  cellular [compartment marker] of plastid stroma in higher plants and cytoplasm in cyanobacteria

355 €

AS01 017  clonality: polyclonal  |  host: hen  |  reactivity: [global antibody] for higher plants, algae, cyanobacteria  |  cellular [compartment marker] of plastid stroma

174 €
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AS01 017S  |  protein standard for quantitation and positive control

When you buy this product you get a 50% discount on other protein standards.
Follow this link to a list of discounted standards.
To get this discount please send your order to orders@agrisera.com

371 €

AS12 2111 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, Z. mays, Ch. reinhardtii

867 €

AS15 2994 | reactivity: Rubisco protein

114 €

AS03 037PRE | host: rabbit

174 €
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AS15 2873S |  protein standard for quantitation and positive control

272 €
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AS14 2775 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana, Vigna radiata