H3K18me3 | Histone H3 (trimethylated Lys18)
AS16 3183 | clonality: polyclonal | host: rabbit | reactivity: chicken, C.elegans, D. melanogaster, human, mouse, plant, rat, Xenopus sp.
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|Recommended dilution||2-5 µg/million cells (ChIP), 1: 1000 (Dot), 1: 50 (IF), 1: 50 (IHC), 1: 500 (WB)|
|Expected | apparent MW||
|Predicted reactivity||C.elegans, chicken, D. melanogaster, mouse, plant, rat, Xenopus sp.|
|Not reactive in||No confirmed exceptions from predicted reactivity known at the moment|
|Additional information||To be added when available|
|Selected references||To be added when available, antibody released in February 2016.|
Dot Blot: using anti-H3K18me3 antibody. Lane 1: K18 unmodified. Lane 2: K18Me1. Lane 3: K18Me2. Lane 4: K18Me3. Lane 5: K18Ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 0.5 μg/ml for 45 min at 4°C. Secondary antibody: Dylight®488 rabbit secondary antibody at 1:10 000 for 45 min at RT.
Immunofluorescence: using anti-H3K18me3 antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10 000 for 45 min at RT. Localization: Histone H3K18me3 is nuclear and chromosomal. Staining: Histone H3K18me3 is expressed in green, nuclei are counterstained with DAPI (blue).
Western Blot: using anti-H3K18me3 antibody. 30 μg of C. elegans embryo lysate. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT. Predicted/Observed size: ~15 kDa.
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