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DMPO | DMPO nitrone adduct

383 €

AS11 1632   | clonality: monoclonal  |  host:mouse |  reactivity:DMPO

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AS11 1632

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product information
Background

DMPO (5,5-dimethly-1-proline N-oxide) is a compound used in spin trapping. It is a method employed in chemistry as an analytical technique for detection and identification of short-lived free radicals. DMPO adducts can be generated with protein and DNA radicals.

Immunogen 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to ovalbumin
Host Mouse
Clonality Monoclonal
Clone IgG1 (clone N1664A)
Purity
Format Liquid
Quantity 100 ĩl
Reconstitution
Storage

store at -20°C for one year. Avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA),immuno-spin trapping (IT), western blot (WB), immunocytochemistry (ICC), immunoprecipitation (IP)
Related products

collection of antibodies to proteins involved in oxidative stress

Secondary antibodies

Additional information

Antibody is purified on Protein G and present in TBS pH 7.4 with 0.05 % sodium azide as preservative and 50 % glycerol.

DMPO antibody in dilution of 1: 1000 was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100 ng/lane by colormetric immunoblot analysis using goat anti-mouse IgG HRP conjugated secondary antibody.

application information
Recommended dilution

1-10 µg/ml with standard ECL (WB), 1-10 µg/ml (ELISA), 10 µg/ml (ICC), 25 µg (IP)

Expected | apparent MW

90 kDa

Confirmed reactivity

DMPO (species independent)

Predicted reactivity DMPO (species independent)
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information

Important note for immunoprecipitation: do not link antibody to matrix prior to use as this causes large loss of reactivity to DMPO adduct.

Selected references

Chatterjee et al. (2009). Immuno-spin trapping of a post-translational carboxypeptidase B1 radical formed by a dual role of xanthine oxidase and endothelial nitric oxide synthase in acute septic mice. Free Radic. Med. and Biol. 46:454-461.



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