HSP101 | ClpB heat shock protein, C-terminal
AS08 287 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, A. tequilana, C. sativus
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1: 1000 with standard ECL (WB)
|Expected | apparent MW||
Arabidopsis thaliana, Agave tequilana, Cucumis sativus L. var Krak
Citrus sinensis, Glycine max, Nicotiana tabacum, Oryza sativa, Populus trichocarpa, Triticum aestivum, Vitis vinifera
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
|Additional information||When testing a new species with this antibody perform a following experiment:
prepare a leaf extract from a leaf without stress and one that has been heat stressed as follows: Take a whole leaf (in the case of plants with small leaves, like Arabidopsis thaliana), or part of a leaf and place on wet filter paper in a petri dish. Heat stress in the dark at 38°C/90 minutes to 2 hours and then allow to recover for 2 hours at room temperature in low light (leave it on the lab bench). You should be able to load 10 ug for western blotting and compare the non-heat stressed to the heat stressed sample.
Use Arabidopsis thaliana leaf as a positive control. Following such treatment 1 ug of a total protein from Arabidopsis thaliana allows detection of HSP101.
|Selected references||Janicka-Russak et al. (2013). Modification of plasma membrane proton pumps in cucumber roots as an adaptation mechanism to salt stress. J Pant Physiol. March 14.
Janicka-Russak et al. (2012). Different effect of cadmium and copper on H+-ATPase activity in plasma membrane vesicles from Cucumis sativus roots. J. Exp. Botany, March 2012, ahead of print.
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