HSP16.6 | Class I heat shock protein 16.6 (cytosolic)
AS08 286 | clonality: polyclonal | host: rabbit | reactivity: Synechocystis sp. PCC 6803
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1: 5000 with standard ECL (WB)
|Expected | apparent MW||
Synechocystis sp. PCC 6803
Synechococcus sp., Thermosynechococcus sp.
|Not reactive in||
no confirmed exceptions from predicted reactivity known in the moment
on a gradient gel Hsp16.6 runs closer to 11 kDa
|Selected references||Gunnelius et al. (2014). The omega subunit of the RNA polymerase core directs transcription efficiency in cyanobacteria. Nucleic Acids Res. 2014 Jan 29.|
Left panel: purified recombinant Hsp16.6 in 0.5, 1 and 2 ng per well. Right panel: 5 µg of total protein from Synechocystis PCC 6803 null mutant and WT were separated on 9-17 % SDS-PAGE and blotted 1h to nitrocellulose (Biorad). Blots were incubated in the primary antibody at a dilution of 1: 5 000 for 1h at room temperature with agitation and a secondary HRP-conjugated antibody (1: 10 000 from Abcam).
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