HSP18.5 | class IV heat shock protein
AS11 1628 | clonality: polyclonal | host: rabbit | reactivity:Arabidopsis thaliana
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1 : 1000 with standard ECL (WB)
|Expected | apparent MW||
|Predicted reactivity||dicots including: Glycine max, Medicago truncatula, Medicago sativa, Pisum sativum, Ricinus communis, Rosa chinensis, monocots including: Zea mays|
|Not reactive in||
no confirmed exceptions from predicted reactivity are currently known
hsp 18.5 is a low abundancy protein and estimated concentration of this protein in total cell extract is ca. 0.007-0.01 %. Therefore to be able to visualize this protein the load per well needs to be at least 20 ug of heat shocked total protein/well. It is crucial to heat treat the plants at 38ºC for 3 hours at high humidity.
Please, note that longer transfer time might result in losing a signal for this protein.
to be added when available.
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