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Soy protein

350 €

AS09 569  |  clonality: polyclonal  |  host: hen  reactivity: Glycine max

PRODUCT INFORMATION IN PDF

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AS09 569

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product information
Background

Soybean (Glycine max) is a plant from legume family with a broad use in food industry.

Immunogen

Glycine max flour protein extract

Host Chicken
Clonality Polyclonal
Clone
Purity Affinity purified IgY
Format Liquid at 1,2 µg/µl in PBS pH 7.2 + 0.1 % sodium azide
Quantity 100 ĩg
Reconstitution
Storage

store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA), Western blot (WB)
Related products

antibodies to food proteins

Plant protein extraction buffer

Secondary antibodies

Additional information

Antibodies were purified on immobilized soybean proteins.

application information
Recommended dilution

0.1-1 µl/ml  (WB), 2- 5 µl/ml  (capture ELISA)

Expected | apparent MW
Confirmed reactivity soybean proteins
Predicted reactivity

soybean proteins

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

to be added when available

Selected references

to be added when available 


application example

western blot detection using anti-soy protein antibodies 1 µg (2) and 5 µg (3) of soy protein extract were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-soy protein antibody (AS09 569, 1:10 000, 0.5 µl/5ml, 1h) and secondary anti-chicken (1:25 000, 1 h) antibody (HRP conjugated, secondary antibody AS09 602) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with TMA-6 (Lumigen) using a Fuji LAS-3000 CCD (300s, standard sensitivity). Exposure time was 2 min. 





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