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Clathrin heavy-chain 1,2 (40 ĩg, HRP-conjugated)

406 €

AS10 690-HRP | clonality: polyclonal | host: rabbit | reactivity: Arabidopis thaliana, Chlamydomonas reinhardtii, Nicotiana tabacum

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AS10 690-HRP

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product information
Background

Clathrin is a protein involved in intracellular trafficking and plays a major role in the formation of coated vesicles. It consists of three clathrin heavy chains and three light chains. Clathrin-coated vesicles (CCV) selectively sort cargo at the cell membrane, trans-Golgi network, and endosomal compartments for multiple membrane traffic pathways.

 

Immunogen

KLH-conjugated peptide derived from available plant clathrin heavy chain sequences including Arabidopsis thaliana clathrin heavy chain 1 UniProt: Q0WNJ6, TAIR:At3g11130, clathrin heavy chain 2 UniProt: Q0WLB5,TAIR:At3g08530

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Liquid, conjugated to HRP
Quantity 40 µg
Reconstitution
Storage

Store at +4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.

Tested applications western blot (WB), immunolocalization (IL), immunofluorescence (IF)
Related products

AS10 680 | Tubulin alpha chain

AS10 681 | Tubulin beta chain

Plant and algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1: 2000 with standard ECL (WB), 1: 2400 (IL), 1:400 (IF)

Expected | apparent MW

193 | 170 kDa (Arabidopsis thaliana)

Confirmed reactivity

Arabidopsis thaliana, Chlamydomonas reinhardtii, Nicotiana tabacum

Predicted reactivity

Amborella trichopoda, Brassica napus, Capsella rubella, Citrus aurantium var. sinensis, Eucalyptus grandisGlycine max, Chlorella variabilisLeucaena glauca, Lotus japonicusMedicago tribuloidesMimulus guttatus, Musa malaccensisOryza sativa, Panicum italicum, Physcomitrella patens, Phaseolus vulgaris, Pisum sativum, Populus balsamifera, Ricinus communis, Selaginella moellendorffii, Sisymbrium salsugineum, Solanum lycopersicum, Theobroma cacao, Triticum aestivum, Vitis vinifera

Not reactive in

Nicotiana benthamiana

Additional information

Please, note that fresh samples will provide better restuls (see image below).

Selected references


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