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95 kDa Lcm (phycobilisome - to- thylakoid core linker with phycocyanobilin chromophore)

345 €

AS08 281  |  clonality:polyclonal  |  host:rabbit  |  reactivity:cyanobacteria and red algae

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AS08 281

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product information
Background

A large polypeptide Lcm with a few phycocyanobilin chromophores which acts as a terminal energy acceptor and as a linker polypeptide between the phycobilisomes and the photosynthetic reaction centres (PS2).

Immunogen

native protein purified from phycobilisomes of Porphyridium cruentum

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS08 277 | anti-APC | allophycocyanin alpha and beta

AS08 278 | anti-R-PC | R-phycocyanin

AS08 279 | anti-b-PE | phycoerythrobilin

AS08 280 | anti-B-PE | phycoerythrobilin and phycourobilin

Additional information

Anabaena sp. sample can serve as a negative control, as under nitrogen deficient conditions phycobiliproteins are going to be lost.
Overall sample quality is of crucial importance and in older or not properly stored samples, phycobiliproteins will undergo proteolytic degradation.

application information
Recommended dilution

1:300 - 1: 1000 with standard ECL (WB)

Expected | apparent MW

95 kDa (Porphyridium cruentum), 75-120 kDa (other species)

Confirmed reactivity Anabaena variablis, Aphanathece halophytica, Fremyella diplosiphon (Microchaete diplosiphon CCALA 811), Mastigocladus laminosus, Nostoc sp., Synechococcus sp. PCC 7002, ... ATCC 27264, ...PR6) , Synechococcus elongatus (strain PCC 7942), Anacystis nidulans R2), Synechococcus leopolensis (..elangatus PCC 6301), Synechecoccus lividus, Spirulina platensis (Athrospira platensis), Tolypothrix tenuis; red alga: Porphyridium cruentum (purpureum), P. aeruginuso, Rhodosorus marinus, Griffithsia monilis
Predicted reactivity

other cyanobacteria and red alge

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information
Selected references Redlinger & Gantt (1982) A Mr 95,000 polypeptide in Porphyridium cruentum phycobilisomes and thylakoids: PNAS 79:5542.
Redlinger & Gantt (1981). Phycobilisome structure of Porphyridium cruentum. Plant Physiol. 68:1375.


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