IKP

PS | Phytochelatin Synthase

Product no: AS20 4489
AS20 4489 | Clonality: Monoclonal | Host: Mouse | Reactivity: Schizosaccharomyces pombe
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  • Product Info
  • Sub class: IgG2a
    Immunogen: N-terminal peptide conjugated to MAP (Multiple Antigenc Peptide)
    Host: Mouse
    Clonality: Monoclonal
    Format: Protein G purified immunoglobulin in PBS pH 7.4, 150 mM NaCl, 0.05 % sodium azide
    Quantity: 100 µg
    Storage: Store at  -20°C. Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Tested applications: ELISA (ELISA), Immunoflourescence (IF), Western blot (WB)
    Recommended dilution: 1: 1000 (ELISA), (WB)
    Expected | apparent MW: 57 kDa
  • Reactivity
  • Confirmed reactivity: Schizosaccharomyces pombe
    Not reactive in: No confirmed exceptions from predicted reactivity known at the moment.
  • Additional Information
  • Additional information (application): This antibody recognized yeast PS overexpressed in Arabidopsis thaliana.
  • Background
  • Background: Together with gamma –glutamyl Cys sythetase (γ-ECS) and glutathione synthetase, phytochelatin synthetase (PS), is an enzyme involved in, and required for, synthetizing phytochelatins (PC). In certain plants, deficiency of such enzymes leads to low tolerance to metal contamination while mutants over-expressing them are highly tolerant to metal contamination and may be used in phyto-remediation of heavy metals.
  • Product Citations
  • Selected references: Chen et al (2005). Overexpression of phytochelatin synthase in Arabidopsis leads to enhanced arsenic tolerance and cadmium hypersensitivity. Plant Cell Physiol. 2004 Dec;45(12):1787-97. Erratum in: Plant Cell Physiol. 46(2):387.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection

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