ABA | Abscisic acid (C1) (for immunolocalization)

270 €

AS09 446 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana, Eucalyptus globulus, Pinus radiata


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AS09 446

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product information

Abscisic acid (ABA) is a plant hormone involved in different physiological responses as stimulation of the closure of stomata (water stress brings about an increase in ABA synthesis), iInhibition of shoot growth, and many others. ABA shown to have both inhibitory as well as many promoting functions.


BSA-conjugated abscisic acid (C1) via C1 carboxyl group

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS pH 7.4. 
Format Liquid containing 50% glycerol
Quantity 200 µg

Store at -20°C; avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Immunofluorescence (IF), Immunolocalization (IL)
Related products

AS06 195 | anti-ABA

AS09 422 | anti-ABA | abscisic acid (C1) (quantity 1mg)

AS09 447 | anti-ABA | abscisic acid (C1) (quantity 5mg)

collection of antibodies to other plant hormones

Secondary antibodies

Additional information

useful references for immunolocalization work:
DeWitte et al. (1999). Dynamics of cytokinins in apical shoot meristems of a day neutral tobacco during floral transition and flower formation. Plant Physiol. 119:111-121.
Shiozaki et al. (1999). Localisation of abscisic acid in grape berry by immunohitochemical techniques. J.Japan Hort.Sci.68:1-9. 
Skene et al. (1987). Model systems for the immunolocalisation of cis, trans-abscisic acid in plant tissues. Planta 172:192-199.
Sosountozov et al. (1986). Immunoelectron-microscopy localisation of abscisic acid with collodial gold on Lowicryl-embeded tissues of Chenopodium polyspermum L. Planta 168: 471-481.
Zavala et al. (1983). Localisation of a phytohormone using immunocytochemistry. J.Cell Biol. 97:1235-1239.

application information
Recommended dilution

to be determined by end user

Expected | apparent MW
Confirmed reactivity abscisic acid (C1) in Arabidopsis thaliana, Eucalyptus globulus, Pinus radiata
Predicted reactivity

abscisic acid (C1)

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information The antibody will recognize either the ABA conjugated to glucose ester (ABA-GE) or the ABA precursor: abscisic acid aldehyde. ABA aldehyde is however not usually present in plant tissue similarly to ABA alcohol which is also reactive. The antibodies will predominantly recognize only ABA and its glucosylester.

for ELISA kit - please inquire

Selected references Escandón et al. (2016). Integrated physiological and hormonal profile of heat-induced thermotolerance in Pinus radiata. Tree Physiol. 2016 Jan;36(1):63-77. doi: 10.1093/treephys/tpv127. Epub 2016 Jan 12.
Ondzighi-Assoume et al. (2016). Environmental Nitrate Stimulates Root Tip Abscisic Acid Accumulation via Release from Inactive Stores. Plant Cell. 2016 Feb 17. pii: TPC2015-00946-RA.
Jesus et al. (2015). Salicylic acid application modulates physiological and hormonal changes in Eucalyptus globulus under water deficit. Environ and Exp Botany, Volume 118, October 2015, Pages 56–66.
Lacuesta et al. (2013). Immunolocalization of IAA and ABA in roots and needles of radiata pine (Pinus radiata) during drought and rewatering. Tree Physiol. May;33(5):537-49.

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