IAA | Indole 3 acetic acid
AS06 193 | clonality: polyclonal | host: rabbit
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|Recommended dilution||1 : 5000-1 : 10 000 (ELISA), 1 : 100-1 :600 (IL)|
|Expected | apparent MW|
|Confirmed reactivity||Arabidoppsis thaliana, Euphorbia pulcherrima, Medicago sativa (nodules)|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
For detailed immunolocalization protocol check here.
|Selected references||Lu et al. (2015). OsPIN5b modulates rice plant architecture and yield by changing auxin homeostasis, transport and distribution. Plant J. 2015 Jul 25. doi: 10.1111/tpj.12939.
Bianco and Defez (2009). Medicago truncatula improves salt tolerance when nodulated by an indole-3-acetic acid-overproducing Sinorhizobium meliloti strain.J Exp. Bot. 60, No. 11: 3097-3107.
4 days old Arabidopsis thaliana seedlings were treated with 1µM NPA for 24 hours. Seedlings were fixed for 20 min in 4% EDAC in MTSB, and next 30 minutes in 4 % EDAC+ 2% Formaldehyde. Anti-auxin anti-rabbit primary antibody (Agrisera, catalog number AS06 193) diluted 1: 600 plus Goat anti-rabbit IgG (H&L), DyLight® 549 Conjugate (AS09 633) as secondary antibody diluted in 1: 3000 (shown in red color) were used.
Courtesy Dr. Taras Pasternak, Freiburg University, Germany.
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