FVIIa | Coagulation factor VIIa (50 ĩg)

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AS00 001  |  clonality: polyclonal  |  host: rabbit  |  reactivity: human


47 st
Item No:
AS00 001

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product information
Background Factor VIIa (FVIIa) is a key serine protease involved in the initiation of the coagulation cascade. FVIIa requires tissue factor (TF), a membrane bound protein, as an essential cofactor for maximal activity towards its biological substrates Factor X, Factor IX and Factor VII (FVII).
Immunogen Recombinant human Factor VIIa (NovoSeven ®, Novo Nordisk  A/S Denmark)
Host Rabbit
Clonality Polyclonal
Purity Affinity purified IgG in PBS pH 7.4
Format Lyophilized in PBS pH 7.4
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them, to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Tested applications Prothrombin assay with human plasma (PT), thrombin generation assay, inhibition assay with Factor VIIa with TF and a chromogenic substrate, inDirect ELISA (I-ELISA)
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Secondary antibodies

Additional information No significant difference in binding to Factor VII and Factor VIIa.

Antibody is purified to total IgG fraction, followed by purification on specific FVIIa column.
application information
Recommended dilution 1 : 15 000 (I-ELISA), 14 µg/ml (IL)
Expected | apparent MW

52 kDa (this is MW of Factor VIIa)

Confirmed reactivity Human
Predicted reactivity Primates
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information This antibody is purified using Protein G, to obtain a total IgG fraction.
Selected references Lopez-Vilchez et al. (2009). Traffic of rFVIIa through Endothelial Cells and Redistribution into Subendothelium: Implications for a Prolonged Hemostatic Effect. Journal of Coagulation Disorders, October 1: (1). (immunolocalization)

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