3-nitroY | nitrotyrosine (100 µl)

345 €

AS10 706-100 | clonality: monoclonal | host: mouse | reactivity:nitrotyrosine

product information

background

Nitrotyrosine is a marker of NO-dependent oxidative stress. It is a product of tyrosine nitration mediated by reactive nitrogen species.

immunogen

BSA-conjugated 3-(4-Hydroxy-3-nitrophenyl acetamido) propionic acid

antibody format

mouse

monoclonal

IgG2A

liquid

quantity

100 µl

storage

store at -20°C; avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

western blot (WB), ELISA (ELISA), immunohistochemistry (IHC), immunoprecipitation (IP)

related products

AS10 706-25 | anti-nitrotyrosine mouse antibody, 25 µg

additional information

1 mg/ml of Protein G purified IgG2A in PBS pH 7.4, 0.09 % sodium azide, 50 % glycerol

application information
recommended dilution		needs to be determined for each application
expected \| apparent MW		n.a.
confirmed reactivity		human, mouse, rat, dog
predicted reactivity		higher plants
not reactive in		no confirmed exceptions from predicted reactivity known in the moment
additional information		antibody recognizes 3-nitrotyrosine moieties. No detectable crossreactivity with non-nitrated tyrosine. Not species specific. 0.7μg/ml was sufficient for detection of 5 μg SIN-1 treated BSA by Western Blot using standard ECL
selected references		Antibody used in western blot and ELISA: Girault et al. (2001).Immunodetection of 3-nitrotyrosine in the liver of zymosan-treated rats with a new monoclonal antibody: comparison to analysis by HPLC. Free Radical Biology and Medicine, 31 (11): 1375-1387. Gow et al. (2004).Biological significance of nitric oxide-mediated protein modifications. Am J Physiol Lung Cell Mol Physiol. 287(2): L262-8. Antibody used in immunohistochemistry: Pfister et al. (2002). Inducible nitric oxide synthase and nitrotyrosine in listeric encephalitis: a cross-species study in ruminants. Vet Pathol. 39: 190-199.

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